Mouse Anti-RPS6KA1 Recombinant Antibody (clone 10H12)
CAT#: MOB-2731z
This product is a mouse antibody that recognizes human RPS6KA1. It can be used in the following applications: WB, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/21_H4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117676-RPS6KA1/subcellular
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Specifications
- Immunogen
- Human Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human RPS6KA1
- Species Reactivity
- Human
- Clone
- 10H12
- Applications
- WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunohistochemistry.
Target
- Alternative Names
- RPS6KA1; MAPKAP Kinase 1a; RSK1; MAPK-Activated Protein Kinase 1a; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; P90RSK1; P90Rsk; EC 2.7.11.1; RSK-1; S6K-Alpha 1; P90S6K; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha-1; Ribosomal Protei
- UniProt ID
- Q15418
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RPS6KA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0827MZ | Recombinant Mouse Anti-Human RPS6KA1 Antibody (clone 8C12) | ELISA | Mouse antibody |
| MRO-2372-CN | Recombinant Rabbit Anti-RPS6KA1 (phosphorylated Ser380) Monoclonal Antibody (CBACN-658) | WB, IF, IP | Rabbit IgG |
| MRO-2373-CN | Recombinant Rabbit Anti-RPS6KA1 (phosphorylated Thr359+S363) Monoclonal Antibody (CBACN-659) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-FY1281 | Recombinant Rabbit Anti-RPS6KA1 (phospho Thr359/Ser363) Antibody (clone R01-5B1) | WB, IP | Rabbit IgG |
| VS3-WK2122 | Rabbit Anti-RPS6KA1 (pS380) Recombinant Antibody (clone R03-8I3) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3121 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA1 Monoclonal Antibody (DS3121AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY227 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (CBACN-658) | IP, Protein Purification | |
| VS-0724-YC1347 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (VS-0724-YC1347) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1018 | CytoStream™ Rabbit Anti-RPS6KA1 Recombinant Antibody (VS13-YC1018) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6219 | Anti-RPS6KA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6220 | Anti-Mouse RPS6KA1 Immunohistochemistry Kit | IHC |
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