Hi-Affi™ Recombinant Rabbit Anti-SYNGAP1 Monoclonal Antibody (DS3480AB)
CAT#: MOR-3480
Recombinant Rabbit anti-SYNGAP1 monoclonal antibody for WB, FC, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197283-SYNGAP1/subcellular
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Specifications
- Immunogen
- A synthetic peptide corresponding to residues on the C terminus of human SynGAP.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS3480AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Flow Cytometry; Immunocytochemistry/Immunofluorescence
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Specific SynGAP1 Detection
This rabbit monoclonal antibody is excellent for detecting SynGAP1 in Western blots. We saw a clean band at the expected size. It is great for our research on synaptic proteins.
Works In ICC
We used this antibody for immunocytochemistry on neuronal cultures. The staining of the postsynaptic density was very clear. The recombinant technology ensures we get consistent performance between lots.
Q&As
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Is this a recombinant antibody?
A: Yes, this is a Hi-Affi™ Recombinant Rabbit Anti-SYNGAP1 Monoclonal Antibody. The use of recombinant technology avoids traditional ascitic fluid approaches and ensures greater specificity, sensitivity, and consistency in large-scale production.
-
What applications are recommended for this antibody?
A: We recommend this antibody for Western Blot (WB), Flow Cytometry (FC), and Immunocytochemistry/Immunofluorescence (ICC/IF). It targets SYNGAP1, a major component of the postsynaptic density associated with NMDA receptors.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SYNGAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1612 | Rabbit Anti-SYNGAP1 Recombinant Antibody (clone R03-4F4) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1134 | Rabbit Anti-SYNGAP1 Recombinant Antibody (VS13-YC1134) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7049 | Anti-Mouse SYNGAP1 Immunohistochemistry Kit | IHC |
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