Rabbit Anti-SYNGAP1 Recombinant Antibody (clone R03-4F4)
CAT#: VS3-FY1612
This anti-SynGAP antibody detected positive in western blot analysis of SynGAP in human and rat brain lysates. The recombinant monoclonal antibody has been shown to be effective to target human and rat SYNGAP1. The R03-4F4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38373/165584_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197283-SYNGAP1/subcellular
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WB
Figure 1 Anti-SYNGAP1 Antibody (VS3-FY1612) in WB.
The SynGAP expression in rat Brain lysates were detected using the anti-SYNGAP1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human SynGAP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat SYNGAP1
- Species Reactivity
- Human, Rat
- Clone
- R03-4F4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 148 kDa, while the observed molecular weight stands at 148 kDa.
- Related Disease
- Intellectual Developmental Disorder, Autosomal Dominant 5 and Syngap1-Related Intellectual Disability
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- SYNGAP1; MRD5; RASA1; RASA5; SYNGAP; synaptic Ras GTPase activating protein 1
- Gene ID
- 8831
- UniProt ID
- Q96PV0
- Protein Refseq
- NP_001123538.1; NP_006763.2
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SYNGAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3480 | Hi-Affi™ Recombinant Rabbit Anti-SYNGAP1 Monoclonal Antibody (DS3480AB) | WB, FC, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1134 | Rabbit Anti-SYNGAP1 Recombinant Antibody (VS13-YC1134) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7049 | Anti-Mouse SYNGAP1 Immunohistochemistry Kit | IHC |
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