Chimeric Anti-Siglec15 Recombinant Antibody (TAB-445CT) (CAT#: TAB-445CT)
This product is a recombinant chimeric antibody that recognizes Siglec-15. Siglec-15 was found to be expressed in several cancer types, in particular ovarian cancer, renal cancer, cancer of the central nervous system, and prostate cancer. Recombinant human Siglec-15 was used as an immunogen for the mAb generation.
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SDS-PAGE
Figure 1 Chimeric Anti-SIGLEC15 Recombinant Antibody (TAB-445CT) in SDS-PAGE
SDS-PAGE analysis of TAB-445CT in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
WB
Figure 2 Chimeric Anti-SIGLEC15 Recombinant Antibody (TAB-445CT) in WB
Western blot analysis of TAB-445CT was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-445CT and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
DB
Figure 3 Chimeric Anti-SIGLEC15 Recombinant Antibody (TAB-445CT) in DB
Dot Blot analysis of TAB-445CT was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).
TAB-445CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 4 Chimeric Anti-SIGLEC15 Recombinant Antibody (TAB-445CT) in ELISA
ELISA analysis of TAB-445CT was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with TAB-445CT. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Recombinant human Siglec-15
- Host Species
- Chimeric
- Type
- Chimeric antibody (mouse/human)
- Specificity
- Human Siglec-15
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Blocking
- Related Disease
- Acute myeloid leukaemia
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SIGLEC15; sialic acid binding Ig-like lectin 15; CD33L3; HsT1361; SIGLEC-15; sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
- Gene ID
- 284266
- UniProt ID
- Q6ZMC9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-568CL | Human Anti-SIGLEC15 Recombinant Antibody (TAB-568CL) | ELISA | Human IgG2 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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