Hi-Affi™ Recombinant Rabbit Anti-TARDBP Monoclonal Antibody (DS3504AB)
CAT#: MOR-3504
Recombinant Rabbit anti-TARDBP monoclonal antibody for WB, IHC, ICC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/138_C12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/662_G6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human epididymis
Immunohistochemical staining of human epididymis shows distinct positivity in both nuclei and cytoplasm of glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/166_C12_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_6_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120948-TARDBP
❮
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS3504AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry; Immunocytochemistry; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2370-VHH | Recombinant Anti-Human TARDBP VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0283CL | Mouse Anti-TARDBP Recombinant Antibody (TAB-0283CL) | WB, IF | Mouse IgG |
| TAB-0283CL-S(P) | Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (TAB-0283CL-S(P)) | WB, ELISA | Mouse scFv |
| TAB-0283CL-F(E) | Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (TAB-0283CL-F(E)) | WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-568CT | Human Anti-TARDBP Recombinant Antibody (TAB-568CT) | ELISA, FC, WB | Human IgG |
| TAB-569CT | Human Anti-TARDBP Recombinant Antibody (TAB-569CT) | ELISA, FC, WB | Human IgG |
| TAB-570CT | Human Anti-TARDBP Recombinant Antibody (TAB-570CT) | ELISA, FC, WB | Human IgG |
| TAB-571CT | Human Anti-TARDBP Recombinant Antibody (TAB-571CT) | ELISA, FC, WB | Human IgG |
| TAB-572CT | Human Anti-TARDBP Recombinant Antibody (TAB-572CT) | ELISA, FC, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0148-YC-S(P) | Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0148-YC-S(P)) | ELISA, FC, RIA | Mouse scFv |
| HPAB-0149-YC-S(P) | Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0149-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0150-YC-S(P) | Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0150-YC-S(P)) | ELISA, Block | Mouse scFv |
| NS-091CN-S(P) | Rabbit Anti-TARDBP Recombinant Antibody; scFv Fragment (NS-091CN-S(P)) | ELISA, IHC, IP, ICC, IF | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0148-YC-F(E) | Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0148-YC-F(E)) | ELISA, FC, RIA | Mouse Fab |
| HPAB-0149-YC-F(E) | Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0149-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0150-YC-F(E) | Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0150-YC-F(E)) | ELISA, Block | Mouse Fab |
| NS-091CN-F(E) | Rabbit Anti-TARDBP Recombinant Antibody; Fab Fragment (NS-091CN-F(E)) | ELISA, IHC, IP, ICC, IF | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-091CN | Rabbit Anti-TARDBP Recombinant Antibody (NS-091CN) | ELISA, IHC, IP, ICC, IF | Rabbit IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1478-CN | Recombinant Rabbit Anti-TARDBP Monoclonal Antibody (JM51-10) | WB, IP, IF, IHC, FC | Rabbit IgG |
| VS3-FY1426 | Recombinant Rabbit Anti-TARDBP Antibody (clone R02-2A8) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-WK1938 | Recombinant Rabbit Anti-TARDBP Capture Antibody (clone R98-7T-3) | ELISA | Rabbit IgG |
| VS7-0425-WR948 | Mouse Anti-TARDBP Recombinant Antibody (clone 7F9A6) | FC | Mouse IgG1 |
| VS7-0425-WR961 | Mouse Anti-TARDBP Recombinant Antibody (clone 6G2D8) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7093 | Anti-TARDBP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7094 | Anti-Mouse TARDBP Immunohistochemistry Kit | IHC | |
| VS-0525-XY7095 | Anti-Zebrafish TARDBP Immunohistochemistry Kit | IHC |
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