Mouse Anti-TARDBP Recombinant Antibody (TAB-0283CL)

Name: Mouse Anti-TARDBP Recombinant Antibody (TAB-0283CL)
SKU: TAB-0283CL
Rating: 5 (1 reviews)

CAT#: TAB-0283CL

This product is a recombinant mouse antibody that recognizes TDP-43. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/138_C12_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/662_G6_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human epididymis

Immunohistochemical staining of human epididymis shows distinct positivity in both nuclei and cytoplasm of glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human pancreas

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/166_C12_1_red_green.jpg

Normal Tissue Figure 6 Cerebral cortex

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_B_8_5.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_5.jpg

Normal Tissue Figure 10 Testis

Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_6_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120948-TARDBP

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human TDP-43

Species Reactivity
Human

Applications
WB, IF

Related Disease
TDP-43 proteinopathy lesion, amyotrophic lateral sclerosis

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TARDBP; TAR DNA binding protein; ALS10; TDP-43; TAR DNA-binding protein 43; TAR DNA-binding protein-43

Gene ID
23435

UniProt ID
A0A024R4E2

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0283CL.pdf

MSDS

MSDS - TAB-0283CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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TAB-0283CL-S(P)	Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (TAB-0283CL-S(P))	WB, ELISA	Mouse scFv
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TAB-568CT	Human Anti-TARDBP Recombinant Antibody (TAB-568CT)	ELISA, FC, WB	Human IgG
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HPAB-0148-YC	Mouse Anti-TARDBP Recombinant Antibody (HPAB-0148-YC)	ELISA, FC, RIA	Mouse IgG2a, κ
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HPAB-0148-YC-S(P)	Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0148-YC-S(P))	ELISA, FC, RIA	Mouse scFv
HPAB-0149-YC-S(P)	Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0149-YC-S(P))	ELISA	Mouse scFv
HPAB-0150-YC-S(P)	Mouse Anti-TARDBP Recombinant Antibody; scFv Fragment (HPAB-0150-YC-S(P))	ELISA, Block	Mouse scFv
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CAT	Product Name	Application	Type
HPAB-0148-YC-F(E)	Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0148-YC-F(E))	ELISA, FC, RIA	Mouse Fab
HPAB-0149-YC-F(E)	Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0149-YC-F(E))	ELISA	Mouse Fab
HPAB-0150-YC-F(E)	Mouse Anti-TARDBP Recombinant Antibody; Fab Fragment (HPAB-0150-YC-F(E))	ELISA, Block	Mouse Fab
NS-091CN-F(E)	Rabbit Anti-TARDBP Recombinant Antibody; Fab Fragment (NS-091CN-F(E))	ELISA, IHC, IP, ICC, IF	Rabbit Fab

CAT	Product Name	Application	Type
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