Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ)

Name: Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ)
SKU: TAB-334CQ
Rating: 5 (1 reviews)

CAT#: TAB-334CQ

This product is a recombinant mouse antibody that recognizes TL1A (Tumor necrosis factor-like ligand 1A). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression Tested Data

Subcellular Location Figure 1 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & nuclear membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46522/1383_A7_2_selected.jpg

Normal Tissue Figure 2 IHC staining of human esophagus

Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_4_1_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_9_5.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_8_3.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_8_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_6_6.jpg

Normal Tissue Figure 7 Skin

Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_B_5_8.jpg

Normal Tissue Figure 8 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12948/29318_A_9_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced.

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181634-TNFSF15

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SDS-PAGE

Figure 1 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in SDS-PAGE

SDS-PAGE analysis of TAB-334CQ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-334CQ was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 2 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in HPLC

The purity of TAB-334CQ was 99.0172% as determined by SEC-HPLC.

Figure 3 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in WB

Western blot analysis of TAB-334CQ was performed by loading recombinant human TNFSF15 protein.

TAB-334CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ) in ELISA

ELISA analysis of TAB-334CQ was performed by coating with recombinant human TNFSF15 protein. Then blocked with BSA, and incubated with Mouse Anti-TNFSF15 Recombinant Antibody (TAB-334CQ). The Goat Anti-Mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Specifications

Immunogen
Human recombinant TL1A

Host Species
Mouse

Type
Mouse IgG2a, κ

Specificity
Human TL1A (Tumor necrosis factor-like ligand 1A)

Species Reactivity
Human

Applications
ELISA

Related Disease
Asthma, inflammatory bowel disease

Product Property

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TNFSF15; Tumor necrosis factor superfamily member 15; TL1; TL1A; VEGI; VEGI192A; tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A

Gene ID
9966

UniProt ID
O95150

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-334CQ.pdf

MSDS

MSDS - TAB-334CQ.pdf

COA

Certificate of Analysis Lookup

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TAB-334CQ-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody; Fab Fragment (TAB-334CQ-F(E))	ELISA, FC, BL	Mouse Fab
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CAT	Product Name	Application	Type
TAB-340CQ-F(E)	Human Anti-TNFSF15 Recombinant Antibody; Fab Fragment (TAB-340CQ-F(E))	ELISA, FC, Neut	Human Fab
TAB-341CQ-F(E)	Human Anti-TNFSF15 Recombinant Antibody; Fab Fragment (TAB-341CQ-F(E))	ELISA, FC, Neut	Human Fab
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TAB-343CQ-F(E)	Human Anti-TNFSF15 Recombinant Antibody; Fab Fragment (TAB-343CQ-F(E))	ELISA, FC, Neut	Human Fab
TAB-344CQ-F(E)	Human Anti-TNFSF15 Recombinant Antibody; Fab Fragment (TAB-344CQ-F(E))	ELISA, FC, Neut	Human Fab

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CAT	Product Name	Application	Type
HPAB-N0287-YC	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-N0287-YC)	Inhib, Neut	Mouse IgG
HPAB-N0288-YC	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-N0288-YC)	Inhib, Neut	Mouse IgG
HPAB-N0289-YC	Human Anti-TNFSF15 Recombinant Antibody (HPAB-N0289-YC)	Inhib, Neut	Humanized IgG
HPAB-N0290-YC	Human Anti-TNFSF15 Recombinant Antibody (HPAB-N0290-YC)	Block	Human IgG
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CAT	Product Name	Application	Type
HPAB-0831-FY-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0831-FY-F(E)); Fab Fragment	Inhib, ELISA	Mouse Fab
HPAB-0832-FY-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0832-FY-F(E)); Fab Fragment	Inhib, ELISA	Mouse Fab
HPAB-0833-FY-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0833-FY-F(E)); Fab Fragment	Inhib, ELISA	Mouse Fab
HPAB-0834-FY-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0834-FY-F(E)); Fab Fragment	Inhib, ELISA	Mouse Fab
HPAB-1443-FY-F(E)	Mouse Anti-TNFSF15 Recombinant Antibody; Fab Fragment (HPAB-1443-FY-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
HPAB-0831-FY-S(P)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0831-FY-S(P)); scFv Fragment	Inhib, ELISA	Mouse scFv
HPAB-0832-FY-S(P)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0832-FY-S(P)); scFv Fragment	Inhib, ELISA	Mouse scFv
HPAB-0833-FY-S(P)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0833-FY-S(P)); scFv Fragment	Inhib, ELISA	Mouse scFv
HPAB-0834-FY-S(P)	Mouse Anti-TNFSF15 Recombinant Antibody (HPAB-0834-FY-S(P)); scFv Fragment	Inhib, ELISA	Mouse scFv
HPAB-0455-WJ-F(E)	Human Anti-TNFSF15 Recombinant Antibody; Fab Fragment (HPAB-0455-WJ-F(E))	ELISA	Humanized Fab

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