Human Anti-TRIM28 Recombinant Antibody (clone D530)
CAT#: DrMAB-622
Recombinant Human Antibody binds selectively to Human TRIM28, expressed in E. coli.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/1132_B11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10066/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_9_7_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_4_6.jpg
Normal Tissue
Figure 9 Skin
Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_1.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130726-TRIM28
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human TRIM28
- Species Reactivity
- Human
- Clone
- D530
- Applications
- ELISA, IP-MS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- TRIM28; tripartite motif containing 28; KAP1; TF1B; RNF96; TIF1B; PPP1R157; transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interact
- Gene ID
- 10155
- UniProt ID
- Q13263
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-054 | Recombinant Anti-human TRIM28 VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
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| MOB-2165MZ | Recombinant Mouse Anti-Human TRIM28 Antibody | IHC-P, WB | Mouse antibody |
| VS3-QX1166 | Mouse Anti-TRIM28 Recombinant Antibody (clone 4E1-D12-F8) | WB, ICC, IHC, IP | Mouse IgG1 |
| VS3-CJ66 | Mouse Anti-TRIM28 Recombinant Antibody (VS3-CJ66) | WB, ELISA | Mouse IgG |
| VS3-WK926 | Rabbit Anti-TRIM28 Recombinant Antibody (VS3-WK926) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1488 | Recombinant Rabbit Anti-TRIM28 Antibody (clone R07-5E6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3681 | Rabbit Anti-TRIM28 Recombinant Antibody (clone DS3681AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1372 | AbPlus™ Anti-TRIM28 Magnetic Beads (VS-0724-YC1372) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7525 | Anti-TRIM28 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7526 | Anti-Mouse TRIM28 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7527 | Anti-Bovine TRIM28 Immunohistochemistry Kit | IHC |
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