Mouse Anti-TRIM28 Recombinant Antibody (clone 4E1-D12-F8)
CAT#: VS3-QX1166
This product is a mouse antibody that recognizes TRIM28. The antibody can be used in applications: WB, ICC, IHC, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/1132_B11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10066/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_9_7_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_4_6.jpg
Normal Tissue
Figure 9 Skin
Fibroblasts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Keratinocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Langerhans
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Melanocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_B_7_1.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64033/142911_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130726-TRIM28
❮
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Specifications
- Immunogen
- Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- KAP1/TIF1β
- Species Reactivity
- Human
- Clone
- 4E1-D12-F8
- Applications
- WB, ICC, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl), 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TRIM28"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-622 | Human Anti-TRIM28 Recombinant Antibody (clone D530) | ELISA, IP-MS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-054 | Recombinant Anti-human TRIM28 VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2165MZ | Recombinant Mouse Anti-Human TRIM28 Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3681 | Rabbit Anti-TRIM28 Recombinant Antibody (clone DS3681AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0892-CN | Rabbit Anti-TRIM28 Recombinant Antibody (clone CBACN-336) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2242-CN | Rabbit Anti-TRIM28 Polyclonal Antibody (MRO-2242-CN) | WB, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ66 | Mouse Anti-TRIM28 Recombinant Antibody (VS3-CJ66) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1372 | AbPlus™ Anti-TRIM28 Magnetic Beads (VS-0724-YC1372) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7525 | Anti-TRIM28 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7526 | Anti-Mouse TRIM28 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7527 | Anti-Bovine TRIM28 Immunohistochemistry Kit | IHC |
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