Anti-VEEV E2 Recombinant Antibody (CUF37-2a) (CAT#: MRO-1163LC)

Figure 1 Reactivity of CUF37-2a to multiple VEEV strains.

CUF37-2a (20 μg/ml) was tested by ELISA using antigen prepared from VEEV strains TC-83, TrD, P676, 3880, Mena II, 78V, Fe37c, BeAn8, Pixuna, CaAr508 and AG80 (subtypes IA/B, IA/B, IC, ID, IE, IF, II, IIIA, IV, V and VI respectively).

Figure 2 CUF37-2a reacts with the VEEV E2 glycoprotein.

HEK 293 cells were transfected with plasmids expressing either the E2 or E1 glycoprotein of VEEV. 48 h later, the cells were fixed and reacted with 10 μg/ml CUF37-2a, 1A3B7 (E2-specific) or 3B2A9 (E1-specific) followed by anti-mouse IgG-FITC. The first two columns show representative fields of view under UV illumination. The third column shows identical brightfield views of negative UV-illuminated views.

Figure 1 Anti-VEEV E2 Antibody (CUF37-2a) (MRO-1163LC) in ELISA

ELISA analysis of MRO-1163LC was performed by coating with VEEV E2 glycoprotein.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)

Figure 2 Anti-VEEV E2 Antibody (CUF37-2a) (MRO-1163LC) in WB

Western blot analysis of MRO-1163LC was performed with VEEV E2 glycoprotein.
MRO-1163LC incubation concentration: 2ng/μL.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

Figure 3 Anti-VEEV E2 Antibody (CUF37-2a) (MRO-1163LC) in Dot Blot

Dot Blot analysis of MRO-1163LC was performed by coating with VEEV E2 glycoprotein.
MRO-1163LC incubation concentration: 2ng/μL.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)