HRP-conjugated Rabbit Anti-Mouse IgG polyclonal Antibody
CAT#: MOB-0225MC
HRP-conjugated Rabbit anti-Mouse IgG polyclonal antibody
Specifications
- Immunogen
- Mouse IgG whole molecule
- Host Species
- Rabbit
- Species Reactivity
- Mouse
- Conjugate
- HRP
Product Property
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Format
- Liquid
- Concentration
- 2 mg/ml (Please refer to the vial label for the specific concentration)
- Buffer
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, 0.01% Sodium Azide, pH7.4, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Applications
- Application Notes
- ELISA 1:6,000 - 1:30,000 WESTERN BLOT 1:3,000 - 1:15,000 IMMUNOHISTOCHEMISTRY 1:1,500 - 1:7,500
Target
- Alternative Names
- IgG; Immunoglobulin G
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Reliable HRP Conjugate
This HRP-conjugated Rabbit Anti-Mouse IgG is a workhorse in our lab for Western Blotting. It provides strong signal amplification and clean backgrounds. The immunoaffinity purification removes unwanted reactivities, making it highly specific for mouse IgG. A very dependable secondary antibody.
Excellent For IHC
We used this antibody for Immunohistochemistry (IHC-P) at a dilution of 1:2000. The staining was crisp and specific. It is stabilized with BSA and preservatives, making it robust. We keep the concentrated solution at 4°C for daily use and it performs consistently.
Q&As
-
How is this secondary antibody purified?
A: This Rabbit Anti-Mouse IgG antibody is prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. This is followed by solid phase adsorption to remove unwanted reactivities.
-
What are the recommended dilutions?
A: For ELISA, we recommend a dilution of 1:6,000 - 1:30,000. For Western Blot, 1:3,000 - 1:15,000, and for Immunohistochemistry, 1:1,500 - 1:7,500. These are guidelines, and optimal dilutions should be determined experimentally.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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