Anti-FcRH5 (clone 10A8)-SPDB-DM4 ADC
CAT#: ADC-172CL
This ADC product is composed of an anti-FcRH5 antibody (clone 10A8) conjugated via SPDB linker to DM4(Hu anti-FcRH5 10A8-SPDB-DM4). It has demonstrated a response in treatment by a MOA (Mechanism of Action) as microtubule inhibitor.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Group enriched (U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143297-FCRL5
❮
❯
Specifications
- Antibody Overview
- Hu Anti-FcRH5 10A8
- Clone
- 10A8
- Linker
- SPDB (N-succinimidyl-4-(2-pyridyldithio)butyrate)
- Linker Class/Description
- Class: Chemically cleavable linkers-Disulfide Linkers
Description: Disulfide Linkers are extensively exploited as a chemically labile linkage. Since the release of disulfide-linked drugs requires a cytoplasmic thiol cofactor, such as glutathione (GSH). Disulfides maintain stable at physiological pH and only when ADCs are internalized inside cells, the cytosol provides reducing environment including intracellular enzyme protein disulfide isomerase, or similar enzymes, drugs can be released.
- Drug
- DM4 (N2'-Deacetyl-N2'-(4-mercapto-4-methyl-1-oxopentyl)maytansine)
- Drug Class/Description
- Class: Maytansinoid
Description: Maytansinoids are a group of cytotoxins structurally similar to rifamycin, geldanamycin, and ansatrienin. The eponymous natural cytotoxic agent maytansine is a 19-member lactam (ansa
macrolide) structure originally isolated from the Ethiopian shrub Maytenus ovatus. Maytansinoids can bind to tubulin at or near the vinblastine-binding site, which interfere the formation of microtubules and depolymerize already formed microtubules, inducing mitotic arrest in the intoxicated cells.
Target
- Introduction
- This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
- Alternative Names
- FCRL5; Fc receptor-like 5; CD307; FCRH5; IRTA2; BXMAS1; CD307e; PRO820; Fc receptor-like protein 5; fcR-like protein 5; fc receptor homolog 5; immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2);
- Gene ID
- 83416
- UniProt ID
- Q96RD9
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ADC-172CL. Click the button above to contact us or submit your feedback about this product.
Valuable For B Cell Studies
The anti-FcRH5 antibody clone 10A8 has proven to be a highly specific reagent for our B cell development research. The expression of FcRH5 on chromosome 1 makes it an interesting target, and this conjugate helped us visualize the effects of payload delivery in cells expressing this immunoglobulin superfamily receptor.
Cleavable Linker Performance
We were particularly interested in the performance of the SPDB linker in this conjugate. The disulfide linkage demonstrated excellent stability at physiological pH, and we observed efficient release of the DM4 maytansinoid payload upon internalization, which is consistent with the intracellular reduction mechanism we are studying.
Q&As
-
What is the biological function of the target FcRH5?
A: FcRH5 (Fc receptor-like 5) is a member of the immunoglobulin receptor superfamily. It is a single-pass type I membrane protein implicated in B cell development and lymphomagenesis. The protein contains 8 immunoglobulin-like C2-type domains and is clustered on chromosome 1.
-
What type of linker connects the antibody and the payload?
A: The antibody is conjugated via an SPDB (N-succinimidyl-4-(2-pyridyldithio)butyrate) linker. This is a chemically cleavable disulfide linker. It relies on the presence of cytoplasmic thiol cofactors, such as glutathione, to reduce the disulfide bond and release the payload intracellularly.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 10A8"
- CAT
- Product Name
See other products for "FCRL5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2390z | Mouse Anti-FCRL5 Recombinant Antibody (MOB-2390z) | FC, CyTOF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2390z-F(E) | Recombinant Anti-Human FCRL5 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2390z-S(P) | Recombinant Anti-Human FCRL5 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0590CL | Mouse Anti-FCRL5 Recombinant Antibody (TAB-0590CL) | FC, WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0591CL | Mouse Anti-FCRL5 Recombinant Antibody (TAB-0591CL) | FC, IF, WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0592CL | Mouse Anti-FCRL5 Recombinant Antibody (TAB-0592CL) | FC, WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0590CL-S(P) | Mouse Anti-FCRL5 Recombinant Antibody; scFv Fragment (TAB-0590CL-S(P)) | ELISA, FC, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0591CL-S(P) | Mouse Anti-FCRL5 Recombinant Antibody; scFv Fragment (TAB-0591CL-S(P)) | ELISA, FC, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0016CQ | Human Anti-FCRL5 Recombinant Antibody (HPAB-0016CQ) | ELISA, WB, RIA | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0017CQ | Human Anti-FCRL5 Recombinant Antibody (HPAB-0017CQ) | ELISA, WB, RIA | Human IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0018CQ | Human Anti-FCRL5 Recombinant Antibody (HPAB-0018CQ) | RIA, ELISA | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0016CQ-S(P) | Human Anti-FCRL5 Recombinant Antibody; scFv Fragment (HPAB-0016CQ-S(P)) | ELISA, WB, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0017CQ-S(P) | Human Anti-FCRL5 Recombinant Antibody; scFv Fragment (HPAB-0017CQ-S(P)) | ELISA, WB, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0018CQ-S(P) | Human Anti-FCRL5 Recombinant Antibody; scFv Fragment (HPAB-0018CQ-S(P)) | RIA, ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0016CQ-F(E) | Human Anti-FCRL5 Recombinant Antibody; Fab Fragment (HPAB-0016CQ-F(E)) | ELISA, WB, RIA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0017CQ-F(E) | Human Anti-FCRL5 Recombinant Antibody; Fab Fragment (HPAB-0017CQ-F(E)) | ELISA, WB, RIA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0018CQ-F(E) | Human Anti-FCRL5 Recombinant Antibody; Fab Fragment (HPAB-0018CQ-F(E)) | RIA, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-056CQ | Mouse Anti-FCRL5 Recombinant Antibody (MOB-056CQ) | ELISA, FC, FuncS | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0180CQ-F(E) | Human Anti-FCRL5 Recombinant Antibody (clone 9D12); Fab Fragment | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0180CQ-S(P) | Human Anti-FCRL5 Recombinant Antibody (clone 9D12); scFv Fragment | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC616 | Recombinant Anti-FCRL5 Vesicular Antibody, EV Displayed (VS-0425-YC616) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC126 | SmartAb™ Recombinant Anti-FCRL5 pH-dependent Antibody (Clone 8A1) | ELISA, FC | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, IHC, FC, Cyt, ELISA
Application: WB, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: WB, ELISA, FC, IHC, IF, IP
-2.png)
Application: ELISA
Application: ELISA, FuncS
Application: ELISA, WB, IF, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
