I-Ab/Human Eα (ASFEAQGALANIAVDKA) MHC Tetramer
CAT#: MHC-LC703
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of Eα-derived peptide of ASFEAQGALANIAVDKA sequence covering 52-68 and I-Ab molecule. The pMHC tetramer recognizes mouse CD4 T cells, and can be used in the analysis of individual antigen-specific T cells.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_8_3.jpg
Normal Tissue
Figure 3 High expression in lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_7_8.jpg
Normal Tissue
Figure 4 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_5_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, REH, U-266/70, U-698, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204287-HLA-DRA
❮
❯
Specifications
- Allele
- I-Ab
- Class
- Class II
- MHC Species
- Mouse
- Antigen
- Eα
- Antigen Species
- Human
- Peptide
- ASFEAQGALANIAVDKA
- Range
- 52-68
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
- Alternative Names
- HLA-DRA; Major histocompatibility complex, class II, DR alpha; HLA-DRA1
- Gene ID
- 3122
- UniProt ID
- P01903
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MHC-LC703. Click the button above to contact us or submit your feedback about this product.
Excellent E-alpha Tetramer
This I-Ab tetramer loaded with the E-alpha peptide ASFEAQGALANIAVDKA is a standard tool for our antigen presentation studies. It consistently allows us to detect specific CD4 T cells in mice with the Y-Ae antibody or direct tetramer staining applications.
Reliable Control For Presentation
We use this reagent to track the presentation of the E-alpha peptide by I-Ab molecules. The tetramer is stable and provides reproducible results, which is essential for our experiments monitoring antigen processing and MHC class II loading.
Q&As
-
What is the source of the E-alpha peptide in this tetramer?
A: The peptide ASFEAQGALANIAVDKA (residues 52-68) is derived from the E-alpha chain (HLA-DRA). It is a well-studied epitope in the context of antigen processing and presentation by I-Ab molecules in mice.
-
What type of T cells does this I-Ab tetramer detect?
A: As an MHC Class II tetramer (I-Ab), this product is designed to detect antigen-specific CD4 positive T cells. It is widely used to study the Y-Ae epitope and T cell recognition in C57BL/6 mice.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HLA-DRA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2072z | Mouse Anti-HLA-DRA Recombinant Antibody (clone 16F11) | WB, ELISA, FC, ICC, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0310MZ | Recombinant Mouse Anti-Human HLA-DR antigen, Alpha-Chain Antibody (clone UAL.2B6) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1622 | Rabbit Anti-HLA-DRA Recombinant Antibody (clone DS1622AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3350 | PE-DRB1*04:04/Human HLA-DRA (ANIAVDKANLEIMTKR) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-037CN | Human Anti-HLA-DRA Recombinant Antibody (clone HD4) | ELISA, WB, FC, FuncS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-038CN | Human Anti-HLA-DRA Recombinant Antibody (clone HD8) | ELISA, WB, FC, FuncS | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-037CN-F(E) | Human Anti-HLA-DRA Recombinant Antibody (clone HD4); Fab Fragment | ELISA, WB, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-038CN-F(E) | Human Anti-HLA-DRA Recombinant Antibody (clone HD8); Fab Fragment | ELISA, WB, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-037CN-S(P) | Human Anti-HLA-DRA Recombinant Antibody (clone HD4); scFv Fragment | ELISA, WB, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0752-CN | Mouse Anti-HLA-DRA Recombinant Antibody (clone 10-D8) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0753-CN | Rabbit Anti-HLA-DRA Recombinant Antibody (clone CBACN-277) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1936-CN | Rabbit Anti-HLA-DRA Polyclonal Antibody (MRO-1936-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0025-YJ-S(P) | Mouse Anti-HLA-DRA Recombinant Antibody (clone ChDN1924); scFv Fragment | Apop, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0025-YJ-F(E) | Human Anti-HLA-DRA Recombinant Antibody (clone ChDN1924); Fab Fragment | Apop, IF | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY125 | AbPlus™ Anti-HLA-DRA Magnetic Beads (Bra30) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY250 | Mouse Anti-NHP HLA-DRA Recombinant Antibody (clone IPO-10) | IF, FC | Mouse IgG3, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC442 | Recombinant Anti-HLA-DRA Vesicular Antibody, EV Displayed (VS-0425-YC442) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3177 | Anti-HLA-DRA Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3178 | Anti-Sheep HLA-DRA Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3179 | Anti-Rat HLA-DRA Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3180 | Anti-Human HLA-DRA Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3181 | Anti-Mouse HLA-DRA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
