PE-A*02:01/HPV16 E7 (YMLDLQPETT) MHC Tetramer

CAT#: MHC-LC436

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of HPV16-derived peptide of YMLDLQPETT sequence covering 11-20 and A*02:01 molecule. The pMHC tetramer recognizes human CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

Specifications

  • Allele
  • A*02:01
  • Class
  • Class I
  • MHC Species
  • Human
  • Antigen
  • HPV16 E7
  • Antigen Species
  • HPV16
  • Peptide
  • YMLDLQPETT
  • Range
  • 11-20
  • Conjugate
  • PE
  • Application
  • FCM

Target

  • Antigen Introduction
  • Papillomaviridae is an ancient taxonomic family of non-enveloped DNA viruses, collectively known as papillomaviruses. Several hundred species of papillomaviruses, traditionally referred to as "types", have been identified infecting all carefully inspected mammals, but also other amniotes such as birds, snakes and turtles. Infection by most papillomavirus types, depending on the type, is either asymptomatic (e.g. most Beta-PVs) or causes small benign tumors, known as papillomas or warts (e.g. human papillomavirus1, HPV6 or HPV11). Papillomas caused by some types, however, such as human papillomaviruses 16 and 18, carry a risk of becoming cancerous.
  • Alternative Names
  • HPV16 E7; Human papillomavirus type 16 E7
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Breast cancer biomarkers at key points during disease progression
Good For HPV16 E7 Epitope
This PE-labeled A*02:01 tetramer for the longer HPV16 E7 (11-20) peptide worked well. We were able to detect T cells specific for the YMLDLQPETT sequence. The signal was clear and helped us compare responses to different E7 epitopes.
Breast cancer biomarkers at key points during disease progression
Specific Viral Peptide Detection
We used this product to study cellular immunity to the human papillomavirus. The tetramer binding was specific, and the reagent allowed us to accurately enumerate the antigen-specific CD8 T cells in our flow cytometry assays.

Q&As

  1. What is the peptide sequence loaded in this HPV16 E7 tetramer?

    A: The peptide sequence is YMLDLQPETT, which covers amino acids 11-20 of the HPV16 E7 protein. This is slightly longer than the standard 11-19 epitope often used, offering an alternative for T cell studies.

  2. Which fluorophore is conjugated to this tetramer?

    A: This product is conjugated with Phycoerythrin (PE), a fluorescent protein that emits a bright orange/yellow signal, making it highly effective for flow cytometry analysis of antigen-specific T cells.

View the frequently asked questions answered by Creative Biolabs Support.

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