Rat IgG2a, kappa Isotype Control Antibody
CAT#: MOB-211CQ
This product is a Rat IgG2a, kappa isotype control antibody and it can also be used as a negative control in various diagnostic applications such as ICFC, WB, IP, ICC, IF, IHC, FuncS.
Specifications
- Host Species
- Rat
- Type
- Rat IgG2a, κ
- Applications
- Used for immunoassay techniques such as: Intracellular Staining for Flow Cytometry; Western Blot; Immunoprecipitation; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Functional Studies
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Rat IgG2a, kappa Isotype Control; Rat IgG2a, kappa negative control
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Reliable for Experiment Validation
Using this isotype control has significantly increased our confidence in the specificity of our results. It has been instrumental in setting accurate baselines, particularly in flow cytometry, enabling us to distinguish true positive signals from background noise effectively.
Versatile in Various Assays
This isotype control antibody's compatibility with multiple assays like ELISA and immunohistochemistry makes it incredibly versatile. Since adopting it in our lab routines, we've consistently achieved clearer distinctions between non-specific bindings and genuine results.
A Must-Have for Rigorous Testing
For any laboratory engaged in detailed antibody work, this control is indispensable. It's been a reliable part of our experimental setup, ensuring that our findings stand up to scrutiny by clearly identifying background signals in our assays.
Q&As
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What is the primary application of the Rat IgG2a, kappa Isotype Control Antibody?
A: This isotype control antibody primarily serves as a negative control to verify the specificity of primary antibodies in various immunoassays, such as flow cytometry, immunohistochemistry, and immunoprecipitation.
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Is this isotype control antibody purified?
A: Yes, the Rat IgG2a, kappa Isotype Control is highly purified, with purity levels of over 95%, as confirmed through SDS-PAGE analysis.
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Why would a researcher choose a rat-derived isotype control?
A: Rat isotype controls offer compatibility with mouse models, often used in research settings, providing relevant negative controls for studies involving rat or mouse antibodies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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