Mouse Anti-CHKA Recombinant Antibody (clone 19B6)
CAT#: MOB-1594z
This product is a mouse antibody that recognizes human CHKA. It can be used in the following applications: WB, ELISA, IF, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72803/1350_D9_2_selected.jpg
Normal Tissue
Figure 2 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24153/53221_A_8_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24153/53221_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24153/53221_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24153/53221_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110721-CHKA
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Specifications
- Immunogen
- Human Choline Kinase Alpha
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Antibody Isotype
- IgG2b, κ
- Specificity
- Human CHKA
- Species Reactivity
- Human
- Clone
- 19B6
- Applications
- WB, ELISA, IF, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- CHKA; EC 2.7.1.32; CHETK-Alpha; CK; EC 2.7.1.82; EK; Choline Kinase Alpha; CKI; Choline Kinase; CHK; Ethanolamine Kinase
- UniProt ID
- P35790
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Reliable Choline Kinase Detection
We used clone 19B6 to detect Choline Kinase Alpha in our cell lysates. The antibody worked well in Western blot, providing a specific signal that helped us monitor the biosynthesis pathway of phosphatidylcholine in our study.
Consistent ELISA Performance
This mouse IgG2b antibody demonstrated consistent performance in our ELISA assays for CHKA. It binds human Choline Kinase Alpha with good affinity, making it a dependable reagent for our metabolic enzyme research.
Q&As
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What is the target of clone 19B6?
A: Clone 19B6 is a mouse antibody that recognizes Human CHKA (Choline Kinase Alpha). It targets the initial enzyme in the CDP-choline pathway for phosphatidylcholine biosynthesis.
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What is the isotype of this mouse antibody?
A: This is a Mouse IgG2b, kappa antibody. It is greater than 95% pure as determined by SDS-PAGE and has been reported for use in WB, ELISA, IF, and IP applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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