Mouse Anti-SAFB Recombinant Antibody (clone 25G1)
CAT#: MOB-1597z
This product is a mouse antibody that recognizes human SAFB. It can be used in the following applications: WB, ELISA, IF, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16832/132_H11_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20076/if_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in white pulp Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_B_7_4.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160633-SAFB
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Specifications
- Immunogen
- Human Scaffold Attachment Factor B
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human SAFB
- Species Reactivity
- Human
- Clone
- 25G1
- Applications
- WB, ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- SAFB; SAFB1; SAF-B; Heat-Shock Protein (HSP27) Estrogen Response Element And TATA Box-Binding Protein; HSP27 Estrogen Response Element-TATA Box-Binding Protein; SAF-B1; Hsp27 ERE-TATA Binding Protein; HSP27 ERE-TATA-Binding Protein; Glutathione S-Transfer
- UniProt ID
- Q15424
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SAFB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1455MZ | Recombinant Mouse Anti-Human SAFB Antibody (clone 5j60) | ICC, IHC-P, IP, WB | Mouse antibody |
| ZG-079R | Mouse Anti-SAFB Recombinant Antibody (ZG-079R) | WB | Mouse IgG |
| ZG-080R | Mouse Anti-SAFB Recombinant Antibody (ZG-080R) | WB, ICC | Mouse IgG |
| VS3-FY1305 | Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1999 | Anti-SAFB Immunohistochemistry Kit | IHC | |
| VS-0525-XY6336 | Anti-Mouse SAFB Immunohistochemistry Kit | IHC | |
| VS-0525-XY6335 | Anti-Human SAFB Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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