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Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1)

Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) (CAT#: VS3-FY1305)

Name: Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1)
SKU: VS3-FY1305
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes SAFB. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R03-7A1 is specific for human, mouse SAFB. The antigen is synthetic peptide of human safb.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in ICC.

Figure 1. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in ICC.

Immunocytochemical analysis of SAFB1 (green) in 293 using SAFB1 antibody and DAPI (blue).

ICC

Figure 2. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in ICC.

Figure 2. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in ICC.

Immunocytochemical analysis of SAFB1 (green) in 293 using SAFB1 antibody and DAPI (blue).

Figure 3. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in WB.

Figure 3. Recombinant Rabbit Anti-SAFB Antibody (clone R03-7A1) in WB.

Western Blot analysis of S6K1 in rat brain lysates using an S6K1 antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16832/132_H11_1_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20076/if_selected.jpg

Normal Tissue

Figure 3 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_5_6.jpg

Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_8_8.jpg

Figure 5 Spleen
(Cells in red pulp Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in white pulp Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_B_7_4.jpg

Figure 6 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_B_7_5.jpg

Figure 7 Colon
(Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_9_3.jpg

Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_9_4.jpg

Figure 9 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1969/5196_A_7_5.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160633-SAFB

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human SAFB.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse SAFB

Species Reactivity
Human, Mouse

Clone
R03-7A1

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 103 kDa; Observed MW: 150 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Scaffold Attachment Factor B; HSP27 Estrogen Response Element-TATA Box-Binding Protein; Hsp27 ERE-TATA Binding Protein; HSP27 ERE-TATA-Binding Protein; SAF-B1; SAF-B; SAFB1

Gene ID
6294

UniProt ID
Q15424

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products

CAT	Product Name	Application	Type
MOB-1597z	Mouse Anti-SAFB Recombinant Antibody (clone 25G1)	WB, ELISA, IF, IHC	Mouse IgG2b, κ
MOB-1455MZ	Recombinant Mouse Anti-Human SAFB Antibody (clone 5j60)	ICC, IHC-P, IP, WB	Mouse antibody
ZG-079R	Mouse Anti-SAFB Recombinant Antibody (ZG-079R)	WB	Mouse IgG
ZG-080R	Mouse Anti-SAFB Recombinant Antibody (ZG-080R)	WB, ICC	Mouse IgG

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