Recombinant Rabbit Anti-MDK Antibody (clone R06-4B2)
CAT#: VS3-FY916
This product is a recombinant rabbit antibody that recognizes MDK. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R06-4B2 is specific for human MDK. The antigen is human midkine synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57126/996_H3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells and stroma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_4_6.jpg
Normal Tissue
Figure 5 Endometrium
Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (NTERA-2, REH, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110492-MDK
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WB
Figure 1. Recombinant Rabbit Anti-MDK Antibody (clone R06-4B2) in WB.
Western Blot analysis of Midkine in U251, 293 lysates using Midkine Antibody.
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Specifications
- Immunogen
- Human Midkine Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MDK
- Species Reactivity
- Human
- Clone
- R06-4B2
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 16 kDa; Observed MW: 16 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MDK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0812CL | Human Anti-MDK Recombinant Antibody (TAB-0812CL) | ELISA | Humanized antibody |
| TAB-0812CL-S(P) | Human Anti-MDK Recombinant Antibody; scFv Fragment (TAB-0812CL-S(P)) | ELISA | Humanized scFv |
| TAB-0812CL-F(E) | Human Anti-MDK Recombinant Antibody; Fab Fragment (TAB-0812CL-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0813CL | Mouse Anti-MDK Recombinant Antibody (TAB-0813CL) | ELISA, FC, Inhib | Mouse IgG |
| TAB-0813CL-S(P) | Mouse Anti-MDK Recombinant Antibody; scFv Fragment (TAB-0813CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0813CL-F(E) | Mouse Anti-MDK Recombinant Antibody; Fab Fragment (TAB-0813CL-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1922MZ | Recombinant Mouse Anti-Human MDK Antibody (clone TD5) | ELISA | Mouse antibody |
| MRO-0999-CN | Recombinant Rabbit Anti-MDK Monoclonal Antibody (JF096-5) | WB, IHC, IP | Rabbit IgG |
| HPAB-AP735-YC | Human Anti-MDK Recombinant Antibody (clone EV3032) | ELISA, Neut | Human IgG1 |
| HPAB-1953-FY | Mouse Anti-MDK Recombinant Antibody (HPAB-1953-FY) | ELISA, Neut, Inhib | Mouse IgG1 |
| ZG-0464U | Rabbit Anti-MDK Recombinant Antibody (clone 2G3) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2198 | Hi-Affi™ Recombinant Rabbit Anti-MDK Monoclonal Antibody (DS2198AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1029 | PE-A*02:01/Human MDK (AQCQETIRV) MHC Tetramer | FCM, IHC | |
| MHC-CN1030 | APC-A*02:01/Human MDK (AQCQETIRV) MHC Tetramer | FCM, IHC | |
| MHC-CN1031 | FITC-A*02:01/Human MDK (AQCQETIRV) MHC Tetramer | FCM, IHC | |
| MHC-CN1032 | A*02:01/Human MDK (AQCQETIRV) MHC Tetramer | FCM, IHC | |
| MHC-YF562 | A*24:02/Human Midkine (RYNAQCQETI) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP735-YC-S(P) | Human Anti-MDK Recombinant Antibody (clone EV3032); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-1953-FY-S(P) | Mouse Anti-MDK Recombinant Antibody; scFv Fragment (HPAB-1953-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1953-FY-F(E) | Mouse Anti-MDK Recombinant Antibody; Fab Fragment (HPAB-1953-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-AP735-YC-F(E) | Human Anti-MDK Recombinant Antibody (clone EV3032); Fab Fragment | ELISA, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1328 | Anti-MDK Immunohistochemistry Kit | IHC | |
| VS-0525-XY4321 | Anti-Human MDK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC219 | Recombinant Anti-MDK Eliminating Antibody, pH-Sensitive (VS-0625-YC219) | Antigen-Sweeping In Vivo. | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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