Mouse Anti-ACTA2 Recombinant Antibody (clone 2B5)
CAT#: MOB-0257MZ
This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human Smooth Muscle Actin.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3761/630_C6_2_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2/ihc_selected.jpg
Normal Tissue
Figure 3 Breast
Myoepithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_1_4.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166, HHSteC, HSkMC, hTERT-RPE1, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107796-ACTA2
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Specifications
- Immunogen
- N-terminal syntheticdecapeptide of α-smooth muscle actin coupled to keyhole limpet haemocyanin(KLH)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Smooth Muscle Actin
- Species Reactivity
- Human
- Clone
- 2B5
- Applications
- IHC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ACTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4006z | Mouse Anti-ACTA2 Recombinant Antibody (clone 1H7) | ELISA, IHC, WB | Mouse IgG1, κ |
| ZG-0261F | Mouse Anti-ACTA2 Recombinant Antibody (ZG-0261F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0968F | Mouse Anti-ACTA2 Recombinant Antibody (clone 1A4) | EM, ICC, IHC-Fr, IHC-P, WB | Mouse IgG2a |
| ZG-1036F | Mouse Anti-ACTA2 Recombinant Antibody (clone HHF35) | EM, ELISA, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS3-WK830 | Mouse Anti-ACTA2 Recombinant Antibody (clone 1E9) | IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0048 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone DS48AB) | IHC, ICC, FC | Rabbit IgG |
| MOR-4737 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone TH251DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY109 | Anti-ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY110 | Anti-Mouse ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY111 | Anti-Rabbit ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY112 | Anti-Rat ACTA2 Immunohistochemistry Kit | IHC |
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