Recombinant Human Anti-human PAX5 Antibody (CAT#: MHH-752)
Recombinant Human Antibody is against Human PAX5, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-PAX5 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-PAX5 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/2055_D7_2_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/2055_D7_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68498/1354_G10_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68498/1354_G10_4_selected.jpg
Figure 3 IHC staining of human lymph node
(High expression. RNA expression: 39.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_8_8_val_selected.jpg
(High expression. RNA expression: 39.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_8_8_val_selected.jpg
Figure 4 IHC staining of human cerebral cortex
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_8_5_val_selected.jpg
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_8_5_val_selected.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_7_5.jpg
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_A_7_5.jpg
Figure 6 Spleen
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_9_4.jpg
(Cells in red pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56394/140587_B_9_4.jpg
Figure 7 RNA cell line category: Group enriched (Daudi, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196092-PAX5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196092-PAX5
Specifications
- Immunogen
- Human paired box 5
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human paired box 5
- Species Reactivity
- Human
- Applications
- Products can be used for: Enzyme-linked Immunosorbent Assay; Western blot; Immunofluorescence; Functional Study
Product Property
- Purity
- 95% by HPLC
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- PAX5; paired box 5; ALL3; BSAP; paired box protein Pax-5; paired domain gene 5; paired box homeotic gene 5; transcription factor PAX 5; B-cell lineage specific activator
- Gene ID
- 5079
- UniProt ID
- Q02548
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-752-F(E) | Recombinant Anti-human PAX5 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-752-F(E) | Recombinant Human Anti-human PAX5 Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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