Z-domain of protein A scaffold protein anti-Human IgG (CAT#: SZA-L231)

1. Q 1: What does it mean to use protein A scaffold protein's Z-domain for anti-Human IgG detection in research applications?

   A: Protein A's Z-domain binds to the Fc region of human IgG antibodies with great affinity, which makes it an excellent tool for detecting, purifying, and quantifying IgGs in a variety of scientific and medical applications.

2. Q 2: Is there a difference between the binding ability of protein A scaffold protein's Z-domain and its full-length counterpart?

   A: The Z-domain of protein A is engineered for optimal binding to human IgG, often providing comparable if not superior binding capacity to that of full-length protein A, particularly in certain applications where a smaller fragment is advantageous.

3. Q 3: What are some practical factors to take into account while conjugating a protein's Z-domain? An enzyme or fluorescent dye as a reporter molecule and a scaffold protein?

   A: Important factors to consider include the preservation of binding activity after conjugation, optimal conjugation chemistry to avoid steric hindrance, and maintaining the integrity of the Z-domain to ensure effective IgG binding.

View the frequently asked questions answered by Creative Biolabs Support.