Rabbit Anti-ACP5 Recombinant Antibody (clone R06-2R5)
CAT#: VS3-XY3251
This rabbit recombinant antibody specifically recognizes Acid phosphatase 5, tartrate resistant (ACP5), which is a important protein involved in osteopontin/bone sialoprotein dephosphorylation. It reacts with human, mouse and rat ACP5. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lung
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/ihc_selected.jpg
Normal Tissue
Figure 3 Lung
Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_3_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (BEWO, K-562, RPMI-8226, SK-MEL-30, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102575-ACP5
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACP5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-2R5
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Spondyloenchondrodysplasia With Immune Dysregulation; Hairy Cell Leukemia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- HPAP; TRAP; TRAcP; TRACP5a; TRACP5b; TrATPase
- Gene ID
- 54
- UniProt ID
- P13686
- Long Name
- Acid phosphatase 5, tartrate resistant
- Sequence Similarities
- Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Glycosylation at Asn116 and Asn147 (NX_P13686 [NX_P13686-1])
Modification sites at PhosphoSitePlus (P13686)
Glycosylation from GlyGen (P13686) 3 sites
- Protein Refseq
- NP_001104504.1; NP_001104505.1; NP_001104506.1
- Function
- Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACP5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1414CT | Mouse Anti-ACP5 Recombinant Antibody (clone 37F6) | IF, IHC-P | Mouse IgG2b |
| ZG-0145C | Mouse Anti-ACP5 Recombinant Antibody (clone ABT-TRAP) | IHC-P, IF | Mouse IgG |
| VS3-CJ639 | Mouse Anti-ACP5 Recombinant Antibody (VS3-CJ639) | IHC | Mouse IgG2a |
| VS3-XY18 | Mouse Anti-ACP5 Recombinant Antibody (clone 5C5E7) | ELISA, WB, FC | Mouse IgG1 |
| VS3-FY20 | Recombinant Mouse Anti-ACP5 Antibody (clone 8A10-8D6-3A3) | IHC-P | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY89 | Anti-ACP5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY90 | Anti-Mouse ACP5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY91 | Anti-Rat ACP5 Immunohistochemistry Kit | IHC |
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