Rabbit Anti-ACP5 Recombinant Antibody (clone R06-2R5) (CAT#: VS3-XY3251)
This rabbit recombinant antibody specifically recognizes Acid phosphatase 5, tartrate resistant (ACP5), which is a important protein involved in osteopontin/bone sialoprotein dephosphorylation. It reacts with human, mouse and rat ACP5. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/if_selected.jpg
Figure 2 IHC staining of human lung
(Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/ihc_selected.jpg
(Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2584/ihc_selected.jpg
Figure 3 Lung
(Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_3_4.jpg
(Macrophages Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_3_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_9_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59463/139573_A_9_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (BEWO, K-562, RPMI-8226, SK-MEL-30, U-138 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102575-ACP5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102575-ACP5
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACP5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-2R5
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Spondyloenchondrodysplasia With Immune Dysregulation; Hairy Cell Leukemia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- HPAP; TRAP; TRAcP; TRACP5a; TRACP5b; TrATPase
- Gene ID
- 54
- UniProt ID
- P13686
- Long Name
- Acid phosphatase 5, tartrate resistant
- Sequence Similarities
- Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Glycosylation at Asn116 and Asn147 (NX_P13686 [NX_P13686-1])
Modification sites at PhosphoSitePlus (P13686)
Glycosylation from GlyGen (P13686) 3 sites
- Protein Refseq
- NP_001104504.1; NP_001104505.1; NP_001104506.1
- Function
- Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1414CT | Mouse Anti-ACP5 Recombinant Antibody (clone 37F6) | IF, IHC-P | Mouse IgG2b |
| ZG-0145C | Mouse Anti-ACP5 Recombinant Antibody (clone ABT-TRAP) | IHC-P, IF | Mouse IgG |
| VS3-XY18 | Mouse Anti-ACP5 Recombinant Antibody (clone 5C5E7) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY19 | Mouse Anti-ACP5 Recombinant Antibody (clone 7E6A11) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY20 | Recombinant Mouse Anti-ACP5 Antibody (clone 8A10-8D6-3A3) | IHC-P | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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