Mouse Anti-ACTA1 Recombinant Antibody (clone 4G10) (CAT#: VS3-WK1580)

• Immunogen
• Synthetic peptide of Muscle actin

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human ACTA1

• Species Reactivity
• Human, Rat, Mouse

• Clone
• 4G10

• Applications
• IHC-P, IF

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• IHC: 1/100-1/200

• Alternative Names
• Actin, Alpha 1, Skeletal Muscle; Nemaline Myopathy Type 3; ACTA; Actin, Alpha Skeletal Muscle; Alpha-Actin-1; CFTD1; CFTDM; ASMA

• Gene ID
• 58

• UniProt ID
• P68133

• Long Name
• Actin, Alpha 1, Skeletal Muscle

• Sequence Similarities
• Belongs to the actin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton

• Post Translation Modifications
• Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
  Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
  Methylated at His-75 by SETD3.
  (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
  The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

• Protein Refseq
• NP_001091.1

• Function
• Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Secondary Antibody

• 2RD-A1514-YJ
• Sheep Anti-Mouse IgG-heavy and light chain Secondary antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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