Mouse Anti-ACTA2 Recombinant Antibody (clone 4F4)
CAT#: VS3-QX11
This product is a mouse antibody that recognizes ACTA2. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3761/630_C6_2_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2/ihc_selected.jpg
Normal Tissue
Figure 3 Breast
Myoepithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_1_4.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166, HHSteC, HSkMC, hTERT-RPE1, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107796-ACTA2
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Specifications
- Immunogen
- Purified recombinant fragment of human ACTA2 expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- ACTA2
- Species Reactivity
- Human, Monkey
- Clone
- 4F4
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000
Target
REVIEWS AND Q&AS
CITATIONS
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Long Shelf Life
The long shelf life of this antibody makes it a cost-effective choice for our lab. No issues with degradation.
Excellent Technical Support
Whenever we had questions, the technical support was quick and helpful. They guided us through initial setup and troubleshooting.
Cost-Effective
Considering the ease of use and consistent results, it is a highly cost-effective reagent for our research.
Q&As
-
What are the benefits of using this antibody in a multiplex assay?
A: The high specificity and affinity make it suitable for multiplex assays, allowing simultaneous detection of multiple targets without cross-reactivity.
-
Can this antibody be used with both fresh and formalin-fixed samples?
A: Yes, it has been validated for use with both types of samples. Ensure proper protocol adjustments for each sample preparation method.
-
What strategies enhance antibody longevity and stability?
A: Store in aliquots at -20°C or lower, avoid repeated freeze-thaw cycles, and use stabilizers like BSA or glycerol if recommended.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4006z | Mouse Anti-ACTA2 Recombinant Antibody (clone 1H7) | ELISA, IHC, WB | Mouse IgG1, κ |
| MOB-0257MZ | Mouse Anti-ACTA2 Recombinant Antibody (clone 2B5) | IHC | Mouse IgG2a |
| ZG-0261F | Mouse Anti-ACTA2 Recombinant Antibody (ZG-0261F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0182C | Mouse Anti-ACTA2 Recombinant Antibody (clone 1E12) | WB, IHC-P, IF | Mouse IgG |
| ZG-0183C | Mouse Anti-ACTA2 Recombinant Antibody (clone 6A12) | WB, IF, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0048 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone DS48AB) | IHC, ICC, FC | Rabbit IgG |
| MOR-4737 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone TH251DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY109 | Anti-ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY110 | Anti-Mouse ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY111 | Anti-Rabbit ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY112 | Anti-Rat ACTA2 Immunohistochemistry Kit | IHC |
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