Mouse Anti-ACTA2 Recombinant Antibody (clone 6A12) (CAT#: ZG-0183C)
This product is a mouse antibody that recognizes ACTA2. It can be used in a wide variety of applications, such as: WB, IF, IHC-P.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 1 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Hela cell. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). α-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti-Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti-Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
IF
Figure 2 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Human-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 3 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 4 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Rat-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
WB
Figure 5 Mouse Anti-ACTA2 Antibody (clone 6A12) in WB
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using α-SMA monoclonal antibody.
IHC
Figure 6 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse Cecal tissue using α-SMA monoclonal antibody.
IHC
Figure 7 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 8 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 9 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
Specifications
- Immunogen
- Synthetic Peptide of α-SMA
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6A12
- Applications
- WB, IF, IHC-P
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ACTA2"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0257MZ | Mouse Anti-ACTA2 Recombinant Antibody (clone 2B5) | IHC | Mouse IgG2a |
| ZG-0261F | Mouse Anti-ACTA2 Recombinant Antibody (ZG-0261F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0182C | Mouse Anti-ACTA2 Recombinant Antibody (clone 1E12) | WB, IHC-P, IF | Mouse IgG |
| ZG-0297U | Rabbit Anti-ACTA2 Recombinant Antibody (clone M16-L) | IHC-P | Rabbit IgG |
| VS3-QX11 | Mouse Anti-ACTA2 Recombinant Antibody (clone 4F4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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