Mouse Anti-ACTA2 Recombinant Antibody (clone 6A12)
CAT#: ZG-0183C
This product is a mouse antibody that recognizes ACTA2. It can be used in a wide variety of applications, such as: WB, IF, IHC-P.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Hela cell. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). α-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti-Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min). Goat Anti-Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min).
IF
Figure 2 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Human-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 3 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
IF
Figure 4 Mouse Anti-ACTA2 Antibody (clone 6A12) in IF
Immunofluorescence analysis of Rat-liver tissue. 1, α-SMA monoclonal antibody (6A12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
WB
Figure 5 Mouse Anti-ACTA2 Antibody (clone 6A12) in WB
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using α-SMA monoclonal antibody.
IHC
Figure 6 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse Cecal tissue using α-SMA monoclonal antibody.
IHC
Figure 7 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 8 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 9 Mouse Anti-ACTA2 Antibody (clone 6A12) in IHC
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3761/630_C6_2_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2/ihc_selected.jpg
Normal Tissue
Figure 3 Breast
Myoepithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_1_4.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166, HHSteC, HSkMC, hTERT-RPE1, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107796-ACTA2
❮
❯
Specifications
- Immunogen
- Synthetic Peptide of α-SMA
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6A12
- Applications
- WB, IF, IHC-P
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0183C. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ACTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4006z | Mouse Anti-ACTA2 Recombinant Antibody (clone 1H7) | ELISA, IHC, WB | Mouse IgG1, κ |
| VS7-HM22 | Mouse Anti-ACTA2 Recombinant Antibody (clone 4A4) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM23 | Mouse Anti-ACTA2 Recombinant Antibody (clone CBL012HM) | WB, IF, ICC, FCM, ELISA | Mouse IgG |
| VS7-HM24 | Mouse Anti-ACTA2 Recombinant Antibody (clone CBL013HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| VS7-HM25 | Mouse Anti-ACTA2 Recombinant Antibody (clone CBL014HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0048 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone DS48AB) | IHC, ICC, FC | Rabbit IgG |
| MOR-4737 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone TH251DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY109 | Anti-ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY110 | Anti-Mouse ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY111 | Anti-Rabbit ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY112 | Anti-Rat ACTA2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, WB, BLI, SPR
Application: FuncS, Inhib, IP, ELISA
Application: ELISA, SPR, Inhib, FuncS
Application: Neut, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: Neut, ELISA, IF, IP, FuncS, FC
-2.png)
Application: WB, ELISA, FuncS
Application: ELISA, FC, FuncS
Application: ELISA, WB, IF, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
