SmartAb™ Recombinant Anti-ADORA2A pH-dependent Antibody (Clone 864H1)
CAT#: VS-0825-YC211
The anti-ADORA2A pH-dependent antibody (clone 864H1) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human ADORA2A. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for ADORA2A under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from ADORA2A at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. ADORA2A (Adenosine A2A Receptor) is a G protein-coupled receptor that acts as an important immune checkpoint molecule, regulating T-cell function in the tumor microenvironment. It is also a key regulator of brain function, playing a role in motor control and mood.
Gene Expression
Normal Tissue
Figure 1 Caudate nucleus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75997/166267_B_7_7_val_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75997/166267_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75997/166267_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75997/166267_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75997/166267_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, Karpas-707, RPMI-8226, SK-BR-3, U-266/70, U-266/84, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128271-ADORA2A
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ADORA2A
- Species Reactivity
- Human
- Clone
- 864H1
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0825-YC211. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 864H1"
- CAT
- Product Name
See other products for "ADORA2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3693z | Mouse Anti-ADORA2A Recombinant Antibody (clone 31G2) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| PABL-006 | Mouse Anti-ADORA2A Recombinant Antibody (clone Ab2838) | WB, ELISA, IF, FuncS | Mouse IgG |
| HPAB-0046-WJ | Mouse Anti-ADORA2A Recombinant Antibody (HPAB-0046-WJ) | ELISA | Mouse IgG3, κ |
| HPAB-AP567-YC | Mouse Anti-ADORA2A Recombinant Antibody (HPAB-AP567-YC) | ELISA, FC | Mouse IgG2a, κ |
| VS7-HM45 | Mouse Anti-ADORA2A Recombinant Antibody (clone 6A6G11) | ICC, FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-006 | Mouse Anti-ADORA2A Recombinant Antibody (clone Ab2838); scFv Fragment | WB, ELISA, IF, FuncS | Mouse scFv |
| HPAB-0046-WJ-S(P) | Mouse Anti-ADORA2A Recombinant Antibody; scFv Fragment (HPAB-0046-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-AP567-YC-S(P) | Mouse Anti-ADORA2A Recombinant Antibody; scFv Fragment (HPAB-AP567-YC-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-006 | Mouse Anti-ADORA2A Recombinant Antibody (clone Ab2838); Fab Fragment | WB, ELISA, IF, FuncS | Mouse Fab |
| HPAB-0046-WJ-F(E) | Mouse Anti-ADORA2A Recombinant Antibody; Fab Fragment (HPAB-0046-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-AP567-YC-F(E) | Mouse Anti-ADORA2A Recombinant Antibody; Fab Fragment (HPAB-AP567-YC-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0089 | Hi-Affi™ Rabbit Anti-ADORA2A Recombinant Antibody (clone DS89AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC495 | Recombinant Anti-ADORA2A Vesicular Antibody, EV Displayed (VS-0425-YC495) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY191 | Anti-ADORA2A Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA
-3.jpg)
Application: ELISA, FC, IF, WB
Application: WB, ELISA, IF, FuncS, Apop, BA
Application: ELISA, Inhib, FuncS
Application: WB, ELISA, FC, IHC, IF, IP
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC, FuncS
Application: ELISA, WB, IF, FC, IP
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
