Mouse Anti-AGO1/AGO2/AGO3 Recombinant Antibody (clone 0725W38)
CAT#: VS-0725-FY200
This recombinant antibody, clone 0725W38, targets a recombinant protein corresponding to amino acids 47-859 of human Ago2. It is validated for Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), and Western Blot (WB). AGO1/AGO2/AGO3 proteins are argonaute family members that play vital roles in RNA interference (RNAi) and RNA silencing, binding to miRNAs or siRNAs to repress translation. This antibody is a valuable tool for studying gene regulation at the post-transcriptional level.
Specifications
- Immunogen
- A recombinant protein corresponding to amino acids 47-859 of human Ago2.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human AGO1/AGO2/AGO3
- Species Reactivity
- Human
- Clone
- 0725W38
- Applications
- Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Related Disease
- Cancer, Viral infections, Neurodegenerative diseases
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 95 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 5 µg per Immunoprecipitation
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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