Mouse Anti-AGO3 Recombinant Antibody (clone 0725W39)
CAT#: VS-0725-FY201
The Mouse Anti-AGO3 Recombinant Antibody (clone 0725W39) is a recombinant antibody targeting a recombinant protein corresponding to amino acids 1-175 of human Ago3. It is suitable for Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), and Immunoprecipitation (IP). AGO3, an Argonaute family member, plays a role in RNA interference and short-interfering-RNA-mediated gene silencing. This antibody is a useful tool for investigating gene regulation and RNA biology.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytoplasmic bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75436/1616_D10_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48342/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48342/119865_B_9_5.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48342/119865_B_5_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48342/119865_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126070-AGO3
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Specifications
- Immunogen
- A recombinant protein that corresponds to amino acids 1-175 of human Ago3.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human AGO3
- Species Reactivity
- Human
- Clone
- 0725W39
- Applications
- Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Related Disease
- Cancer, Viral infections
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 110 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Specific conditions should be optimized by the end user.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AGO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0526-CN | Rabbit Anti-AGO3 Recombinant Antibody (clone CBACN-209) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0533J | Rabbit Anti-AGO3 Recombinant Antibody (clone 4F7) | ELISA | Rabbit IgG |
| VS3-WK1159 | Rabbit Anti-AGO3 Recombinant Antibody (VS3-WK1159) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY216 | Anti-AGO3 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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