Human Anti-Agr2 Recombinant Antibody (clone CPR02)
CAT#: VS-0524-YC26
The antibody targets the recombinant Agr2 antigen (consisting of amino acid residues 41-175 of Agr2) which was confirmed by ELISA. Immunofluorescence analysis on colon biopsy samples from a healthy donor was performed to verify the ability of CPR02 to recognize the human Agr2 antigen.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7912/1873_D5_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of cell line RT4
Immunofluorescent staining of cell line RT4 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7912/1386_F6_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:Medium
Intensity: Moderate
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7912/24377_A_9_3.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7912/24377_A_3_5.jpg
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Specifications
- Host Species
- Human
- Derivation
- Antibody phage display
- Type
- Human IgG
- Specificity
- Human anterior gradient protein 2 homolog (Agr2)
- Species Reactivity
- Human
- Clone
- CPR02
- Applications
- ELISA, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Anterior Gradient 2, Protein Disulphide Isomerase Family Member; Protein Disulfide Isomerase Family A, Member 17; Secreted Cement Gland Protein XAG-2 Homolog; HAG-2; AG-2; HPC8; AG2; Anterior Gradient 2 Homolog (Xenopus Laevis); Anterior Gradient Protein 2 Homolog
- Gene ID
- 10551
- UniProt ID
- O95994
- Long Name
- Anterior Gradient Protein 2 Homolog (Agr2)
- Sequence Similarities
- Belongs to the AGR family.
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Disulfide bond
- Protein Refseq
- NP_006399.1
- Function
- Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity).
Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| TAB-0707CLV-F(E) | Mouse Anti-AGR2 Recombinant Antibody; Fab Fragment (TAB-0707CLV-F(E)) | ELISA | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0708CLV | Human Anti-AGR2 Recombinant Antibody (TAB-0708CLV) | ELISA | Humanized IgG1, κ |
| TAB-0708CLV-F(E) | Human Anti-AGR2 Recombinant Antibody; Fab Fragment (TAB-0708CLV-F(E)) | ELISA | Humanized Fab |
| TAB-0708CLV-S(P) | Human Anti-AGR2 Recombinant Antibody; scFv Fragment (TAB-0708CLV-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0102 | Hi-Affi™ Rabbit Anti-AGR2 Recombinant Antibody (clone DS102AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0037-CN | Rabbit Anti-AGR2 Recombinant Antibody (clone CBACN-013) | WB, IF, IHC, IP, FC | Rabbit IgG |
| HPAB-N0017-YC | Mouse Anti-AGR2 Recombinant Antibody (clone NOV001YC) | FuncS | Mouse IgG1, κ |
| VS3-CJ196 | Rabbit Anti-AGR2 Recombinant Antibody (VS3-CJ196) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS-0923-FY19 | Recombinant Mouse Anti-AGR2 Antibody (VS-0923-FY19) | ELISA, WB | Mouse IgG1 |
| VS3-FY1628 | Rabbit Anti-AGR2 Recombinant Antibody (clone R04-1H6) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0316-WJ-S(P) | Mouse Anti-AGR2 Recombinant Antibody; scFv Fragment (HPAB-0316-WJ-S(P)) | ELISA, Block | Mouse scFv |
| HPAB-N0017-YC-S(P) | Mouse Anti-AGR2 Recombinant Antibody (clone NOV001YC); scFv Fragment | FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0316-WJ-F(E) | Mouse Anti-AGR2 Recombinant Antibody; Fab Fragment (HPAB-0316-WJ-F(E)) | ELISA, Block | Mouse Fab |
| HPAB-N0017-YC-F(E) | Mouse Anti-AGR2 Recombinant Antibody (clone NOV001YC); Fab Fragment | FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY12 | Mouse Anti-NHP AGR2 Recombinant Antibody (clone 10E2) | WB, IHC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY58 | Anti-AGR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY219 | Anti-Mouse AGR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY218 | Anti-Human AGR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY220 | Anti-Rat AGR2 Immunohistochemistry Kit | IHC |
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