Rabbit Anti-AHR Recombinant Antibody (clone 0725W68)
CAT#: VS-0725-FY235
The Rabbit Anti-AHR Recombinant Antibody (clone 0725W68) is a recombinant antibody targeting a peptide from human AHR. It is suitable for Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). AHR (Aryl Hydrocarbon Receptor) is a ligand-activated helix-loop-helix transcription factor involved in regulating responses to planar aromatic hydrocarbons and xenobiotic-metabolizing enzymes. This antibody is a valuable tool for studying environmental toxicology and gene regulation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5072/if_selected.jpg
Normal Tissue
Figure 2 Placenta
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29723/61916_A_3_7_val_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29722/61951_A_8_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29722/61951_A_9_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (hTCEpi, hTERT-HME1, OE19, RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106546-AHR
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Specifications
- Immunogen
- A peptide from human AHR.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AHR
- Species Reactivity
- Human
- Clone
- 0725W68
- Applications
- Chromatin Immunoprecipitation-Sequencing, Immunofluorescence, Immunoprecipitation, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Molecular Weight
- The molecular weight of the antigen is 96 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 0.5-2 µg/mL
Immunoprecipitation: 2 µg/mL per Immunoprecipitation
Immunofluorescence: 2 µg per ml
Chromatin Immunoprecipitation-Sequencing: 2 µg per Chromatin Immunoprecipitation-Sequencing
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AHR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2225z | Mouse Anti-AHR Recombinant Antibody (clone 36D6) | WB, ICC, IF, IHC, IP | Mouse IgG1 |
| MRO-0123-CN | Rabbit Anti-AHR Recombinant Antibody (clone JM34-10) | WB, IF | Rabbit IgG |
| VS3-FY49 | Recombinant Rabbit Anti-AHR Antibody (clone R07-1H9) | WB | Rabbit IgG |
| VS13-YC34 | Rabbit Anti-AHR Recombinant Antibody (VS13-YC34) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY65 | Anti-AHR Immunohistochemistry Kit | IHC | |
| VS-0525-XY234 | Anti-Mouse AHR Immunohistochemistry Kit | IHC | |
| VS-0525-XY233 | Anti-Human AHR Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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