Rabbit Anti-AKT1S1 Recombinant Antibody (clone R05-3A5) (CAT#: VS3-FY2069)
Clone R05-3A5 is a rabbit recombinant monoclonal antibody that specifically targets human AKT1S1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the PRAS40 antibody was determined using western blot analysis of PRAS40 in Hela, A549, C6 lysates.
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WB
Figure 1 Anti-AKT1S1 Antibody (VS3-FY2069) in WB.
The PRAS40 expression in Hela, A549, C6 lysates were detected using the anti-PRAS40 by western blot assay.
Specifications
- Immunogen
- Synthetic peptides of human PRAS40
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat AKT1S1
- Species Reactivity
- Human, Rat
- Clone
- R05-3A5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Breast Intraductal Papillomatosis and Subependymal Glioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- AKT1 Substrate 1; 40 KDa Proline-Rich AKT Substrate; PRAS40; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); Proline-Rich AKT1 Substrate 1; Lobe
- Gene ID
- 84335
- UniProt ID
- Q96B36
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity (PubMed:12524439, 17386266).
Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation (PubMed:17517883).
Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
(Q96B36-AKTS1_HUMAN)
Modification sites at PhosphoSitePlus (Q96B36)
Modification sites at neXtProt (NX_Q96B36)
Glycosylation from GlyGen (Q96B36) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001092102.1; NP_001092103.1; NP_001265088.1; NP_001265089.1; NP_115751.3
- Function
- Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "AKT1S1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1680CT | Mouse Anti-AKT1S1 Recombinant Antibody (clone 84Q32) | IP, WB | Mouse IgG1 |
| VS3-WK1098 | Mouse Anti-AKT1S1 Recombinant Antibody (VS3-WK1098) | WB | Mouse IgG1, κ |
| VS3-XY3255 | Rabbit Anti-AKT1S1 Recombinant Antibody (clone R06-7C1) | WB, IHC-P, IP, FC | Rabbit IgG |
| VS7-HM67 | Mouse Anti-AKT1S1 Recombinant Antibody (clone 6H1C6) | IHC, ICC | Mouse IgG1 |
| VS7-HM68 | Mouse Anti-AKT1S1 Recombinant Antibody (clone 4A11G7) | FCM | Mouse IgG1 |
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