Rabbit Anti-AKT1S1 Recombinant Antibody (clone R05-3A5) (CAT#: VS3-FY2069)

• Immunogen
• Synthetic peptides of human PRAS40

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat AKT1S1

• Species Reactivity
• Human, Rat

• Clone
• R05-3A5

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 40 kDa.

• Related Disease
• Breast Intraductal Papillomatosis and Subependymal Glioma

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• AKT1 Substrate 1; 40 KDa Proline-Rich AKT Substrate; PRAS40; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); Proline-Rich AKT1 Substrate 1; Lobe

• Gene ID
• 84335

• UniProt ID
• Q96B36

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity (PubMed:12524439, 17386266).
  Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation (PubMed:17517883).
  Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
  (Q96B36-AKTS1_HUMAN)
  Modification sites at PhosphoSitePlus (Q96B36)
  Modification sites at neXtProt (NX_Q96B36)
  Glycosylation from GlyGen (Q96B36) 2 sites, 1 O-linked glycan (2 sites)

• Protein Refseq
• NP_001092102.1; NP_001092103.1; NP_001265088.1; NP_001265089.1; NP_115751.3

• Function
• Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody