Rabbit Anti-AKT1S1 Recombinant Antibody (clone R05-3A5)
CAT#: VS3-FY2069
Clone R05-3A5 is a rabbit recombinant monoclonal antibody that specifically targets human AKT1S1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IP. The binding specificity of the PRAS40 antibody was determined using western blot analysis of PRAS40 in Hela, A549, C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-AKT1S1 Antibody (VS3-FY2069) in WB.
The PRAS40 expression in Hela, A549, C6 lysates were detected using the anti-PRAS40 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
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Specifications
- Immunogen
- Synthetic peptides of human PRAS40
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat AKT1S1
- Species Reactivity
- Human, Rat
- Clone
- R05-3A5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Breast Intraductal Papillomatosis and Subependymal Glioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- AKT1 Substrate 1; 40 KDa Proline-Rich AKT Substrate; PRAS40; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich); Proline-Rich AKT1 Substrate 1; Lobe
- Gene ID
- 84335
- UniProt ID
- Q96B36
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity (PubMed:12524439, 17386266).
Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation (PubMed:17517883).
Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
(Q96B36-AKTS1_HUMAN)
Modification sites at PhosphoSitePlus (Q96B36)
Modification sites at neXtProt (NX_Q96B36)
Glycosylation from GlyGen (Q96B36) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001092102.1; NP_001092103.1; NP_001265088.1; NP_001265089.1; NP_115751.3
- Function
- Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOR-4529 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH37DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4671 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH185DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY282 | Anti-AKT1S1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY283 | Anti-Mouse AKT1S1 Immunohistochemistry Kit | IHC |
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