Rabbit Anti-AKT1S1 Recombinant Antibody (clone R06-7C1)
CAT#: VS3-XY3255
This product is a recombinant rabbit antibody that recognizes AKT1S1 (AKT1 substrate 1), which plays a role in nerve growth factor-mediated neuroprotection. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation and Flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse AKT1S1
- Species Reactivity
- Human, Mouse
- Clone
- R06-7C1
- Applications
- WB, IHC-P, IP, FC
- Related Disease
- Breast Intraductal Papillomatosis; Subependymal Glioma.
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- Lobe; PRAS40
- Gene ID
- 84335
- UniProt ID
- Q96B36
- Long Name
- AKT1 substrate 1
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity.
Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1.
- Protein Refseq
- NP_001092103.1; NP_001092102.1
- Function
- Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AKT1S1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1680CT | Mouse Anti-AKT1S1 Recombinant Antibody (clone 84Q32) | IP, WB | Mouse IgG1 |
| VS3-FY2069 | Rabbit Anti-AKT1S1 Recombinant Antibody (clone R05-3A5) | WB, IP | Rabbit IgG |
| VS3-XY3256 | Rabbit Anti-AKT1S1 Recombinant Antibody (clone R06-8A6) | WB, IP | Rabbit IgG |
| VS7-HM67 | Mouse Anti-AKT1S1 Recombinant Antibody (clone 6H1C6) | IHC, ICC | Mouse IgG1 |
| VS7-HM68 | Mouse Anti-AKT1S1 Recombinant Antibody (clone 4A11G7) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0127 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone DS127AB) | WB, IP | Rabbit IgG |
| MOR-4529 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH37DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4671 | Hi-Affi™ Rabbit Anti-AKT1S1 Recombinant Antibody (clone TH185DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY282 | Anti-AKT1S1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY283 | Anti-Mouse AKT1S1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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