Rabbit Anti-AKT1S1 Recombinant Antibody (clone R06-7C1) (CAT#: VS3-XY3255)
This product is a recombinant rabbit antibody that recognizes AKT1S1 (AKT1 substrate 1), which plays a role in nerve growth factor-mediated neuroprotection. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation and Flow cytometry.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43590/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/ihc_selected.jpg
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine and islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21903/ihc_selected.jpg
Figure 5 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_B_7_5.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_8_3.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64427/150348_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204673-AKT1S1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse AKT1S1
- Species Reactivity
- Human, Mouse
- Clone
- R06-7C1
- Applications
- WB, IHC-P, IP, FC
- Related Disease
- Breast Intraductal Papillomatosis; Subependymal Glioma.
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- Lobe; PRAS40
- Gene ID
- 84335
- UniProt ID
- Q96B36
- Long Name
- AKT1 substrate 1
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated by AKT1; phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity.
Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1.
- Protein Refseq
- NP_001092103.1; NP_001092102.1
- Function
- Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1680CT | Mouse Anti-AKT1S1 Recombinant Antibody (clone 84Q32) | IP, WB | Mouse IgG1 |
| VS3-WK1098 | Mouse Anti-AKT1S1 Recombinant Antibody (VS3-WK1098) | WB | Mouse IgG1, κ |
| VS3-FY2069 | Rabbit Anti-AKT1S1 Recombinant Antibody (clone R05-3A5) | WB, IP | Rabbit IgG |
| VS3-XY3256 | Rabbit Anti-AKT1S1 Recombinant Antibody (clone R06-8A6) | WB, IP | Rabbit IgG |
| VS7-HM67 | Mouse Anti-AKT1S1 Recombinant Antibody (clone 6H1C6) | IHC, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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