Mouse Anti-ALDH1A1 Recombinant Antibody (ZG-0279F)
CAT#: ZG-0279F
This product is a mouse antibody that recognizes ALDH1A1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-ALDH1A1 Recombinant Antibody (ZG-0279F) in WB.
Western blot analysis was performed using ALDH1A1 monoclonal antibodies against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysates.
IHC
Figure 2 Mouse Anti-ALDH1A1 Recombinant Antibody (ZG-0279F) in IHC.
Immunohistochemical analysis of paraffin-embedded cerebellar tissue (left) and lung cancer (right) using ALDH1A1 monoclonal antibody by DAB staining.
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/1584_G10_1_selected.jpg
Normal Tissue
Figure 2 High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/8966_A_1_6_val_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_6_6.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_3_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A549, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165092-ALDH1A1
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Specifications
- Immunogen
- Recombinant fragment of human ALDH1A1 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ALDH1A1
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000.
ELISA: 1/10000
Target
- Alternative Names
- Aldehyde Dehydrogenase 1 Family Member A1; Retinaldehyde Dehydrogenase 1; EC 1.2.1.36; RALDH 1; ALDH-E1; RALDH1; ALHDII; ALDH1; PUMB1; ALDC; Epididymis Secretory Sperm Binding Protein Li 53e; Aldehyde Dehydrogenase 1 Family, Member A1; Aldehyde Dehydrogenase Family 1 Member A1; Aldehyde Dehydrogenase, Liver Cytosolic; ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase; cytosolic
- Gene ID
- 216
- UniProt ID
- P00352
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0567MZ | Mouse Anti-ALDH1A1 Recombinant Antibody (clone nBcdbn 72304) | WB | Mouse IgG |
| MRO-0049-CN | Mouse Anti-ALDH1A1 Recombinant Antibody (clone 8F1) | WB, IHC | Mouse IgG1 |
| MRO-0050-CN | Rabbit Anti-ALDH1A1 Recombinant Antibody (clone CBACN-019) | WB, IHC, IP | Rabbit IgG |
| MRO-1634-CN | Rabbit Anti-ALDH1A1 Polyclonal Antibody (MRO-1634-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1635-CN | Rabbit Anti-ALDH1A1 Polyclonal Antibody (MRO-1635-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0136 | Hi-Affi™ Rabbit Anti-ALDH1A1 Recombinant Antibody (clone DS136AB) | WB, IHC-P | Rabbit IgG |
| MOR-0063-FY | Rabbit Anti-ALDH1A1 Recombinant Antibody (clone AFY0034) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC20 | Rabbit Anti-ALDH1A1 Antibody (VS-0924-YC20) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY87 | Anti-ALDH1A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY292 | Anti-Human ALDH1A1 Immunohistochemistry Kit | IHC |
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