Mouse Anti-ANXA1 Recombinant Antibody (clone ABT-ANXA1) (CAT#: VS3-CJ327)

• Immunogen
• Synthesized peptide derived from human Annexin A1

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human ANXA1

• Species Reactivity
• Human

• Clone
• ABT-ANXA1

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

• Gene ID
• 301

• UniProt ID
• P04083

• Sequence Similarities
• Belongs to the annexin family.

• Cellular Localization
• Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle

• Post Translation Modifications
• Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879).
  Phosphorylated in response to EGF treatment (PubMed:2532504).
  Sumoylated.
  Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26.

• Protein Refseq
• NP_000691.1

• Function
• Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544).
  Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity).
  Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549).
  Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549).
  Has no effect on unstimulated T cells (PubMed:17008549).
  Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660).
  Has high affinity for Ca2+ and can bind up to eight Ca2+ ions (By similarity).
  Displays Ca2+-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678).
  Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
  Annexin Ac2-26:
  Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:22879591, PubMed:15187149, PubMed:25664854).
  Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149).
  Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149).
  Promotes resolution of inflammation and wound healing (PubMed:25664854).
  Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS