Mouse Anti-APOL1 Recombinant Antibody (clone 6D12) (CAT#: VS-0724-FY16)
This mouse monoclonal antibody has a high specificity for human APOL1, showing strong binding affinity as determined by surface plasmon resonance (SPR) analysis, with a KD value of 0.86 nM. The clone 6D12 displays specificity towards APOL1-4, while exhibiting no binding to ApoL5 and ApoL6. The binding epitope of 6D12 is identified as V98-D109.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human vulva/anal skin
(Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18885/ihc_selected.jpg
(Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18885/ihc_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/ihc_selected.jpg
(Immunohistochemical staining of human colon shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_8_5.jpg
Figure 6 Urinary bladder
(Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_5.jpg
(Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_5.jpg
Figure 7 Testis
(Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_6.jpg
(Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, HDLM-2, HHSteC, RPTEC TERT1, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100342-APOL1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100342-APOL1
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human APOL1
- Species Reactivity
- Human
- Clone
- 6D12
- Applications
- Immunofluorescence, Immunoprecipitation
- Related Disease
- Focal Segmental Glomerulosclerosis 4 and Glomerulonephritis
Product Property
- Clonality
- Monoclonal Antibody
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunofluorescence: 2 µg/mL
Target
- Alternative Names
- Apolipoprotein L1; Apolipoprotein L; APOL-I; APO-L; APOL; Apolipoprotein L1-B3; Apolipoprotein L 1; Apolipoprotein L-I; FSGS4
- Gene ID
- 8542
- UniProt ID
- O14791
- Long Name
- Apolipoprotein L1
- Sequence Similarities
- Belongs to the apolipoprotein L family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Phosphorylated by FAM20C in the extracellular medium
Glycosylation at Thr236 and Asn261
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 4 sites, 3 O-linked glycans (3 sites)
- Protein Refseq
- NP_001130012.1; NP_001130013.1; NP_001349856.1; NP_003652.2; NP_663318.1
- Function
- May play a role in lipid exchange and transport throughout the body.
May participate in reverse cholesterol transport from peripheral cells to the liver.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "APOL1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0687MZ | Mouse Anti-APOL1 Recombinant Antibody (clone 2E5) | ELISA, WB | Mouse IgG1 |
| ZG-0302F | Mouse Anti-ApoL1 Recombinant Antibody (ZG-0302F) | WB, ELISA | Mouse IgG |
| VS3-XY86 | Mouse Anti-APOL1 Recombinant Antibody (clone 1D4) | ELISA, WB | Mouse IgG1 |
| VS7-HM121 | Mouse Anti-APOL1 Recombinant Antibody (clone CBL045HM) | WB, ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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