Mouse Anti-APOL1 Recombinant Antibody (clone 6D12)
CAT#: VS-0724-FY16
This mouse monoclonal antibody has a high specificity for human APOL1, showing strong binding affinity as determined by surface plasmon resonance (SPR) analysis, with a KD value of 0.86 nM. The clone 6D12 displays specificity towards APOL1-4, while exhibiting no binding to ApoL5 and ApoL6. The binding epitope of 6D12 is identified as V98-D109.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18885/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows distinct positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_8_5.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56156/121414_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, HDLM-2, HHSteC, RPTEC TERT1, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100342-APOL1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human APOL1
- Species Reactivity
- Human
- Clone
- 6D12
- Applications
- Immunofluorescence, Immunoprecipitation
- Related Disease
- Focal Segmental Glomerulosclerosis 4 and Glomerulonephritis
Product Property
- Clonality
- Monoclonal Antibody
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunofluorescence: 2 µg/mL
Target
- Alternative Names
- Apolipoprotein L1; Apolipoprotein L; APOL-I; APO-L; APOL; Apolipoprotein L1-B3; Apolipoprotein L 1; Apolipoprotein L-I; FSGS4
- Gene ID
- 8542
- UniProt ID
- O14791
- Long Name
- Apolipoprotein L1
- Sequence Similarities
- Belongs to the apolipoprotein L family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Phosphorylated by FAM20C in the extracellular medium
Glycosylation at Thr236 and Asn261
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 4 sites, 3 O-linked glycans (3 sites)
- Protein Refseq
- NP_001130012.1; NP_001130013.1; NP_001349856.1; NP_003652.2; NP_663318.1
- Function
- May play a role in lipid exchange and transport throughout the body.
May participate in reverse cholesterol transport from peripheral cells to the liver.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "APOL1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0687MZ | Mouse Anti-APOL1 Recombinant Antibody (clone 2E5) | ELISA, WB | Mouse IgG1 |
| ZG-0302F | Mouse Anti-ApoL1 Recombinant Antibody (ZG-0302F) | WB, ELISA | Mouse IgG |
| VS3-XY86 | Mouse Anti-APOL1 Recombinant Antibody (clone 1D4) | ELISA, WB | Mouse IgG1 |
| VS7-HM121 | Mouse Anti-APOL1 Recombinant Antibody (clone CBL045HM) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0218 | Hi-Affi™ Rabbit Anti-APOL1 Recombinant Antibody (clone DS218AB) | FC, ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY135 | Anti-APOL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY465 | Anti-Human APOL1 Immunohistochemistry Kit | IHC |
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