AibGenesis™ Rabbit Anti-APPL1 Recombinant Antibody (AibG-Rb-PP28)
CAT#: VS14-YC227
Completing our AI-derived suite, the anti-APPL1 mAb (AibG-Rb-PP28) provides high-affinity targeting and dependable performance for APPL1 detection in human, mouse, and rat. APPL1 is an endosomal adaptor protein that facilitates the recruitment of AKT, coupling Adiponectin and Insulin signaling to glucose metabolism and survival.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/108_C8_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_A_9_3.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11138/29029_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157500-APPL1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- APPL1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-PP28
- Applications
- Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (WB): 1/500-1/2000
Immunohistochemistry (IHC): 1/50-1/200
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- Adaptor Protein, Phosphotyrosine Interacting With PH Domain And Leucine Zipper 1; Adaptor Protein, Phosphotyrosine Interaction, PH Domain And Leucine Zipper Containing 1; Adapter Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; DCC-Interacting Protein 13-Alpha; Dip13-Alpha; APPL; Adaptor Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1;
- Gene ID
- 26060
- UniProt ID
- Q9UKG1
- Long Name
- Adaptor Protein, Phosphotyrosine Interacting With PH Domain And Leucine Zipper 1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "APPL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0221 | Hi-Affi™ Rabbit Anti-APPL1 Recombinant Antibody (clone DS221AB) | WB, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY87 | Recombinant Rabbit Anti-APPL1 Antibody (clone R05-8D5) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY137 | Anti-APPL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY474 | Anti-Mouse APPL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY473 | Anti-Human APPL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY475 | Anti-Rat APPL1 Immunohistochemistry Kit | IHC |
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