Rabbit Anti-ATF5 Recombinant Antibody (clone 9D8) (CAT#: ZG-0554J)
This product is a rabbit antibody that recognizes ATF5.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-ATF5 Antibody (ZG-0554J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue
All lanes: ATF5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
IHC
Figure 2 Rabbit Anti-ATF5 Antibody (ZG-0554J) in IHC
IHC image of ZG-0554J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human ATF5
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF5
- Species Reactivity
- Human, Rat
- Clone
- 9D8
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ATFX; HMFN0395; cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; transcription factor ATFx
- Gene ID
- 22809
- UniProt ID
- Q9Y2D1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0132-CN | Rabbit Anti-ATF5 Recombinant Antibody (clone CBACN-049) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK1222 | Rabbit Anti-ATF5 Recombinant Antibody (VS3-WK1222) | WB, ICC, IF, IHC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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