Rabbit Anti-ATF5 Recombinant Antibody (clone 9D8) (CAT#: ZG-0554J)
This product is a rabbit antibody that recognizes ATF5.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-ATF5 Antibody (ZG-0554J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue
All lanes: ATF5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
IHC
Figure 2 Rabbit Anti-ATF5 Antibody (ZG-0554J) in IHC
IHC image of ZG-0554J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human ATF5
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF5
- Species Reactivity
- Human, Rat
- Clone
- 9D8
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ATFX; HMFN0395; cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; transcription factor ATFx
- Gene ID
- 22809
- UniProt ID
- Q9Y2D1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0132-CN | Rabbit Anti-ATF5 Recombinant Antibody (clone CBACN-049) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK1222 | Rabbit Anti-ATF5 Recombinant Antibody (VS3-WK1222) | WB, ICC, IF, IHC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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