Mouse Anti-ATG5 Recombinant Antibody (clone 3C7A8)
CAT#: VS7-HM159
Mouse Anti-ATG5 Recombinant Antibody (clone 3C7A8) is an mouse IgG2b antibody that recognizes the Human ATG5 by ICC, FCM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42973/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_8.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_B_2_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000057663-ATG5
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Specifications
- Immunogen
- Purified recombinant human ATG5 (AA: 144-275) fragment expressed in E. Coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human ATG5
- Species Reactivity
- Human
- Clone
- 3C7A8
- Applications
- ICC, FCM
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Molecular Weight
- 32.4kDa
- Concentration
- Please refer to the tube label.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| BRD-0058MZ | Chicken Anti-ATG5 Polyclonal IgY | WB | Chicken antibody |
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| VS7-HM161 | Mouse Anti-ATG5 Recombinant Antibody (clone 8E8G6) | WB, ICC, FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY23 | AbPlus™ Anti-ATG5 Magnetic Beads (3C7) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC76 | CytoStream™ Rabbit Anti-ATG5 Recombinant Antibody (VS13-YC76) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY591 | Anti-ATG5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY592 | Anti-Mouse ATG5 Immunohistochemistry Kit | IHC |
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