Mouse Anti-ATP6AP1 Recombinant Antibody (AT695)
CAT#: VS-0326-YC695
The anti-ATP6AP1 recombinant antibody (AT695) reacts with human and murine ATP6AP1. ATP6AP1 is a structural accessory subunit required for the assembly and stability of the V-ATPase (Vacuolar H⁺-ATPase) complex. It is essential for the acidification of lysosomes and endosomes, a process vital for protein degradation, nutrient sensing through mTORC1, and the proper functioning of the secretory pathway. The anti-ATP6AP1 antibody (AT695) has been tested in Enzyme-linked Immunosorbent Assay (ELISA), Flow Cytometry (FC) and Western Blot (WB).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular and glomeruli cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15218/36646_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000071553-ATP6AP1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human and Murine ATP6AP1
- Species Reactivity
- Human, Mouse
- Clone
- AT695
- Applications
- ELISA, FC, WB
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB (1/500-1/2000), ELISA (1/5000-1/20000), FC (1/200-1/400)
Target
- Alternative Names
- ATPase H+ Transporting Accessory Protein 1; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Subunit 1; ATPase, H+ Transporting, Lysosomal Accessory Protein 1; Vacuolar Proton Pump Subunit S1; V-ATPase S1 Accessory Protein; V-ATPase Ac45 Subunit; V-ATPase Subunit S1; Protein XAP-3; ATP6IP1; ATP6S1;
- Gene ID
- 537
- UniProt ID
- Q15904
- Long Name
- ATPase H+ Transporting Accessory Protein 1
- Post Translation Modifications
- Unmodified
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATP6AP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0487MZ | Mouse Anti-ATP6AP1 Recombinant Antibody (clone CBLS-048) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM175 | Mouse Anti-ATP6AP1 Recombinant Antibody (clone 5G12A4) | WB, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM176 | Mouse Anti-ATP6AP1 Recombinant Antibody (clone 3H11B12) | WB, FCM | Mouse IgG1 |
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