Rabbit Anti-ATP6V0D1 Recombinant Antibody (clone R07-1A5) (CAT#: VS3-FY1659)

• Immunogen
• Recombinant human ATP6V0D1 protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat ATP6V0D1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R07-1A5

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 40 kDa.

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• ATPase H+ Transporting V0 Subunit D1; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Member D; ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D1; V-ATPase 40 KDa Accessory Protein; Vacuolar Proton Pump Subunit D 1; 32 KDa Accessory Protein; V-ATPase AC39 Subunit; V-ATPase Subunit D 1; VPATPD; ATP6D

• Gene ID
• 9114

• UniProt ID
• P61421

• Cellular Localization
• Cytoplasmic vesicle, Lysosome, Membrane

• Post Translation Modifications
• Phosphoprotein

• Protein Refseq
• NP_004682.2

• Function
• Subunit of the V0 complex of vacuolar(H⁺)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons.
  V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.
  May play a role in coupling of proton transport and ATP hydrolysis (By similarity).
  In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe²⁺ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
  May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody