Rabbit Anti-BAG3 Recombinant Antibody (clone 3D8) (CAT#: ZG-0566J)
This product is a rabbit antibody that recognizes BAG3.
Specific Inquiry
WB
Figure 1 Rabbit Anti-BAG3 Antibody (ZG-0566J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
All lanes: BAG3 antibody at 0.73μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 KDa
Observed band size: 80 KDa
IHC
Figure 2 Rabbit Anti-BAG3 Antibody (ZG-0566J) in IHC
IHC image of ZG-0566J diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human BAG3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BAG3
- Species Reactivity
- Human
- Clone
- 3D8
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BAG3"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0310CL | Human Anti-BAG3 Recombinant Antibody (TAB-0310CL) | ELISA | Human IgG1 |
| TAB-0310CL-S(P) | Human Anti-BAG3 Recombinant Antibody; scFv Fragment (TAB-0310CL-S(P)) | ELISA | Human scFv |
| TAB-0310CL-F(E) | Human Anti-BAG3 Recombinant Antibody; Fab Fragment (TAB-0310CL-F(E)) | ELISA | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0889MZ | Mouse Anti-BAG3 Recombinant Antibody (clone 5A8) | WB | Mouse IgG2b |
| MRO-0151-CN | Rabbit Anti-BAG3 Recombinant Antibody (clone JA90-53) | WB, IHC, IP | Rabbit IgG |
| HPAB-0257-WJ | Mouse Anti-BAG3 Recombinant Antibody (HPAB-0257-WJ) | IP, IB, IF | Mouse IgG |
| VS3-FY133 | Recombinant Rabbit Anti-BAG3 Antibody (clone R01-3F1) | WB | Rabbit IgG |
| VS3-FY135 | Recombinant Rabbit Anti-BAG3 Antibody (clone R09-2G3) | WB | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0320 | Hi-Affi™ Rabbit Anti-BAG3 Recombinant Antibody (clone DS320AB) | IP, WB | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0257-WJ-S(P) | Mouse Anti-BAG3 Recombinant Antibody; scFv Fragment (HPAB-0257-WJ-S(P)) | ELISA, FC | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0257-WJ-F(E) | Mouse Anti-BAG3 Recombinant Antibody; Fab Fragment (HPAB-0257-WJ-F(E)) | ELISA, FC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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