Rabbit Anti-BANF1 Recombinant Antibody (clone R09-3F2)
CAT#: VS3-FY1621
We demonstrated the assay in western blot analysis of BANF1 in Jurkat lysates. It is a amazing rabbit recombinant monoclonal antibody that is engineered to detect human BANF1. This R09-3F2 antibody is validated for western blot analysis of BANF1 in Jurkat lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39242/if_selected.jpg
Normal Tissue
Figure 2 Skeletal muscle
High expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39242/135600_B_3_6_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows nuclear membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32896/158720_B_4_1_selected.jpg
Normal Tissue
Figure 4 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39242/135600_B_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175334-BANF1
❮
❯
WB
Figure 1 Anti-BANF1 Antibody (VS3-FY1621) in WB.
The BANF1 expression in Jurkat lysates were detected using the anti-BANF1 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human BANF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BANF1
- Species Reactivity
- Human
- Clone
- R09-3F2
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 10 kDa, while the observed molecular weight stands at 10 kDa.
- Related Disease
- Nestor-Guillermo Progeria Syndrome and Vaccinia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Barrier To Autointegration Factor 1; Breakpoint Cluster Region Protein 1; BAF; Barrier-To-Autointegration Factor; D14S1460; BCRP1; BCRG1; NGPS
- Gene ID
- 8815
- UniProt ID
- O75531
- Sequence Similarities
- Belongs to the BAF family.
- Cellular Localization
- Chromosome, Cytoplasm, Nucleus
- Post Translation Modifications
- Acetylation, Phosphoprotein
- Protein Refseq
- NP_001137457.1; NP_003851.1
- Function
- Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA.
Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging.
Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner.
Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface.
Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity.
Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1621. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BANF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0463MZ | Mouse Anti-BANF1 Recombinant Antibody (clone CBLS-035) | IF, IHC-P, WB | Mouse IgG2a |
| MRO-0153-CN | Rabbit Anti-BANF1 Recombinant Antibody (clone JG40-43) | WB, IF, IHC, FC | Rabbit IgG |
| VS13-YC87 | Rabbit Anti-BANF1 Recombinant Antibody (VS13-YC87) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0325 | Hi-Affi™ Rabbit Anti-BANF1 Recombinant Antibody (clone DS325AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY693 | Anti-BANF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY694 | Anti-Mouse BANF1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, WB, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC
-3.jpg)
Application: ELISA, WB
-3.jpg)
Application: IB, FC, ELISA, FuncS
Application: ELISA, Neut, FuncS
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
