Mouse Anti-BCHE Recombinant Antibody (clone mAb2) (CAT#: FAMAB-0031CQ)
Recombinant Mouse Antibody clone mAb2, which is specific to BChE. Monoclonal mAb2 recognized BChE in human, rhesus monkey, horse, cat, and tiger plasma and binds pig and bovine BChE.
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Custom Production
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Immunopurify
Figure 2 BChE immunopurified from 1.5 mL plasma by binding to immobilized monoclonal mAb2 or B2 18-5.
BChE was dissociated from the washed beads with 1% trifluoroacetic acid. The Coomassie stained SDS PAGE gel shows a monomer band for pure BChE at 85 kDa and a dimer band at 170 kDa in lanes 5 and 10. The BChE protein captured by mAb2 in lanes 1–4, and by B2 18-5 in lanes 5–9 has an intense band at 85 kDa for the BChE monomer and a band at 170 kDa for the BChE dimer. The immunopurified BChE has contaminating bands at 55 and 25 kDa. Differences in band intensities are explained by handling losses of 20 μL beads.
Onder, S., Tacal, O., & Lockridge, O. (2018). Delipidation of plasma has minimal effects on human butyrylcholinesterase. Frontiers in pharmacology, 9, 117.
Immunopurify
Figure 3 Comparison of BChE purity following one-step purification on Hupresin or on immobilized monoclonal beads.
The precast 4–20% gradient SDS gel was stained with Coomassie blue. Lanes 1 and 5, BChE eluted from Hupresin with 0.1 M TMA pH 7.5. Lane 2, BChE eluted from monoclonal mAb2 with 1% TFA. Lanes 3 and 7, pure human BChE. Lane 6, BChE eluted from monoclonal B2 18-5 with 1% TFA. Lane 4, molecular weight markers.
Onder, S., David, E., Tacal, O., Schopfer, L. M., & Lockridge, O. (2017). Hupresin retains binding capacity for butyrylcholinesterase and acetylcholinesterase after sanitation with sodium hydroxide. Frontiers in pharmacology, 8, 713.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human butyrylcholinesterase
- Species Reactivity
- Human, Rhesus monkey, Horse, Cat, Tiger, Pig, Bovine
- Clone
- mAb2
- Applications
- ELISA, Immunopurify
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunopurify.
Target
- Alternative Names
- Butyrylcholinesterase; Acylcholine Acylhydrolase; Cholinesterase (Serum) 2; Butyrylcholine Esterase; Pseudocholinesterase; Choline Esterase II; Cholinesterase 1; EC 3.1.1.8; CHE1; EC 3.1.1; CHE2; E1
- Gene ID
- 590
- UniProt ID
- P06276
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-102 | Recombinant Anti-chicken BCHE Antibody | ELISA, WB, FC, FuncS | IgG |
| MOB-0948MZ | Mouse Anti-BCHE Recombinant Antibody (clone 4F9) | ELISA | Mouse IgG1 |
| FAMAB-0028CQ | Mouse Anti-BCHE Recombinant Antibody (clone 11D8) | ELISA, WB, IHC | Mouse IgG1, κ |
| FAMAB-0029CQ | Mouse Anti-BCHE Recombinant Antibody (clone B2 12-1) | ELISA | Mouse IgG1, κ |
| ZG-495C | Mouse Anti-BCHE Recombinant Antibody (clone 14) | ELISA | Mouse IgG1, κ |
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