Mouse Anti-BCHE Recombinant Antibody (clone 11D8) (CAT#: FAMAB-0028CQ)
Recombinant Mouse Antibody clone 11D8, which is specific to BCHE. Monoclonal 11D8 bound pure HuBChE with a KD value in the nanomolar range and 11D8 recognized denatured HuBChE.
Specific Inquiry
ELISA
Figure 2 Human BChE is recognized by monoclonal antibody 11D8
Selectivity of 11D8 for recombinant and wild-type BChE by ELISA (60 min detection)
Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.
IHC
Figure 3 Human BChE is recognized by monoclonal antibody 11D8
Immunohistochemistry of human neuromuscular junction (NMJ). Top, nicotinic acetylcholine receptors are visualized with bungarotoxin (Bung). Bottom, staining with 11D8 labels BChE.
Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.
Figure 4 Human BChE is recognized by monoclonal antibody 11D8
11D8 shifts BChE in sucrose gradient. Sucrose gradient of human PRiMA-BChE complex extracted from COS cells. Binding of 11D8 shifts the peaks of tetramer and dimmer. Wider high-S peak suggests a mixed population of IgG/one tetramer and IgG/two tetramers.
Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.
Figure 5 Human BChE is recognized by monoclonal antibody 11D8
11D8 recognizes BChE expressed by COS cells, anchored by ColQ and PRiMA as documented by confocal microscopy
Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.
WB
Figure 6 Antibodies from BChE knockout mice immunized with mouse BChE.
Western blot of recombinant mouse BChE with sera from mice immunized with recombinant mouse BChE (m1–m3), or from mice immunized with BChE purified from human serum (h1–h6), or with monoclonal antibody produced against human low-glycosylated BChE (11D8). In this experiment, each antibody sample was tested with 75 ng, 38 ng and 8 ng of recombinant mouse BChE loaded onto a denaturing gel. In the 11D8 lane, the band marked with "*" represents 750 ng of protein.
Hrabovska, A., Bernard, V., & Krejci, E. (2010). A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains. PLoS One, 5(9), e12892.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human butyrylcholinesterase
- Species Reactivity
- Human
- Clone
- 11D8
- Applications
- ELISA, WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot and Immunohistochemistry.
Target
- Alternative Names
- Butyrylcholinesterase; Acylcholine Acylhydrolase; Cholinesterase (Serum) 2; Butyrylcholine Esterase; Pseudocholinesterase; Choline Esterase II; Cholinesterase 1; EC 3.1.1.8; CHE1; EC 3.1.1; CHE2; E1
- Gene ID
- 590
- UniProt ID
- P06276
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-102-S(P) | Recombinant Anti-chicken BCHE Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| FAMAB-0028CQ-S(P) | Mouse Anti-BCHE Recombinant Antibody (clone 11D8); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0029CQ-S(P) | Mouse Anti-BCHE Recombinant Antibody (clone B2 12-1); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0030CQ-S(P) | Mouse Anti-BCHE Recombinant Antibody (clone B2 18-5); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0031CQ-S(P) | Mouse Anti-BCHE Recombinant Antibody (clone mAb2); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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