Mouse Anti-BCL2 Recombinant Antibody (VS3-CJ564)
CAT#: VS3-CJ564
This product is a mouse antibody that recognizes human BCL2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55295/1484_G3_6_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_B_3_5.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HL-60, Karpas-707, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171791-BCL2
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Specifications
- Immunogen
- Recombinant protein corresponding to fragment (aa/1-195) BCL2
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human BCL2
- Species Reactivity
- Human
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- Citrate-tris-HCl buffer, pH 7.0.
- Preservative
- 0.02% Proclin 300
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot.
Target
- Alternative Names
- Bcl-2; PPP1R50
- Gene ID
- 596
- UniProt ID
- P10415
- Sequence Similarities
- Belongs to the Bcl-2 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
- Post Translation Modifications
- Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354).
Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354).
In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354).
Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophag (PubMed:10567572, PubMed:18570871).
Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974).
Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138).
Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630).
- Protein Refseq
- NP_000624.2; NP_000648.2
- Function
- Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370).
Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354).
Appears to function in a feedback loop system with caspases (PubMed:11368354).
Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354).
Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974).
May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
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