Mouse Anti-BECN1 Recombinant Antibody (clone 5C2)
CAT#: ZG-0050J
This product is a mouse antibody that recognizes BECN1.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-BECN1 Recombinant Antibody (clone 5C2) in IHC
Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.
IHC
Figure 2 Mouse Anti-BECN1 Recombinant Antibody (clone 5C2) in IHC
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Beclin-1 Mouse mAb diluted at 1:200.
WB
Figure 3 Mouse Anti-BECN1 Recombinant Antibody (clone 5C2) in WB
Western blot analysis of 1) 293T Cell Lysate, 2) C2C12 Cell Lysate, 3) Rat Brain Tissue Lysate using Beclin-1 Mouse mAb diluted at 1:2000.
WB
Figure 4 Mouse Anti-BECN1 Recombinant Antibody (clone 5C2) in WB
Western blot analysis of lysates from 1) 293T Cell Lysate, 2) C2C12 Cell Lysate, 3) Rat Brain Tissue cells, (Green) primary Antibody was diluted at 1:1000, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C1 hour.
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Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3949/1891_D2_8_cr600ff296b7030_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28949/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126581-BECN1
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Specifications
- Immunogen
- Synthetic Peptide of Beclin-1 at AA range of 110-190
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BECN1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 5C2
- Applications
- WB, IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "BECN1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2936z | Mouse Anti-BECN1 Recombinant Antibody (clone 2H3) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-QX116 | Mouse Anti-BECN1 Recombinant Antibody (clone 5H8-7A4-5D7) | IHC | Mouse IgG1 |
| VS4-WK48 | Mouse Anti-BECN1 Recombinant Antibody (VS4-WK48) | ELISA, WB | Mouse IgG1 |
| VS7-HM225 | Mouse Anti-BECN1 Recombinant Antibody (clone CBL069HM) | WB, IHC-p, IF, FCM, ELISA | Mouse IgG |
| VS7-HM226 | Mouse Anti-BECN1 Recombinant Antibody (clone CBL070HM) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0352 | Hi-Affi™ Rabbit Anti-BECN1 Recombinant Antibody (clone DS352AB) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY30 | AbPlus™ Anti-BECN1 Magnetic Beads (VS-0424-XY30) | IP, Protein Purification | |
| VS-0724-YC1274 | AbPlus™ Anti-BECN1 Magnetic Beads (VS-0724-YC1274) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY238 | Anti-BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY756 | Anti-Mouse BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY755 | Anti-Human BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY757 | Anti-Rat BECN1 Immunohistochemistry Kit | IHC |
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