Rabbit Anti-BECN1 Recombinant Antibody (clone R07-2K6) (CAT#: VS3-XY3276)
The rabbit recombinant antibody specifically binds to human BECN1 (Beclin 1), which acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. It also has cross-reactivity against mouse and rat BECN1. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3949/1891_D2_8_cr600ff296b7030_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3949/1891_D2_8_cr600ff296b7030_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28949/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28949/ihc_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_9_8.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_6_6.jpg
Figure 8 Placenta
(Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_2_7.jpg
(Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_2_7.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126581-BECN1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126581-BECN1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BECN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-2K6
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Sphingolipidosis; Pancreatic Ductal Adenocarcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- ATG6; VPS30; beclin1
- Gene ID
- 8678
- UniProt ID
- Q14457
- Long Name
- Beclin 1
- Cellular Localization
- Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus
- Post Translation Modifications
- Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy.
Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes (PubMed:21962518).
Polyubiquitinated at Lys-402 with 'Lys-48'-linkages.
- Protein Refseq
- XP_005257817.1; XP_005257816.1
- Function
- Plays a central role in autophagy.
Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2936z | Mouse Anti-BECN1 Recombinant Antibody (clone 2H3) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1687-CN | Rabbit Anti-BECN1 Polyclonal Antibody (MRO-1687-CN) | IF, IHC | Rabbit IgG |
| MRO-1688-CN | Rabbit Anti-BECN1 Polyclonal Antibody (MRO-1688-CN) | IF, IHC, FC | Rabbit IgG |
| ZG-0329F | Mouse Anti-BECN1 Recombinant Antibody (ZG-0329F) | WB, IP | Mouse IgG |
| ZG-0330F | Mouse Anti-BECN1 Recombinant Antibody (ZG-0330F) | WB | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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