Rabbit Anti-BNIP3 Recombinant Antibody (VS13-YC104) (CAT#: VS13-YC104)
The Rabbit Anti-BNIP3 Recombinant Antibody is specific for human and mouse BNIP3. BNIP3, a mitochondrial protein with a BH3 domain, acts as a pro-apoptotic factor, interacting with anti-apoptotic proteins, with methylation in tumors leading to gene silencing. The anti-BNIP3 antibody can be used for Western blot (1/500-1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Normal Tissue
RNA Expression
Figure 1 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/ihc_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11676/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11676/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_3.jpg
Figure 5 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_7_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_7_4.jpg
Figure 6 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_3_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_3_3.jpg
Figure 7 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_5.jpg
Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_5_6.jpg
Figure 9 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3015/8137_A_8_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (A-431, hTERT-RPE1, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176171-BNIP3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000176171-BNIP3
Specifications
- Immunogen
- Recombinant human BNIP3, aa 90-130.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse BNIP3
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- BCL2 Interacting Protein 3; BCL2/Adenovirus E1B 19kDa Interacting Protein 3; NIP3; BCL2/Adenovirus E1B 19 KDa Protein-Interacting Protein 3;
- Gene ID
- 664
- UniProt ID
- Q12983
- Long Name
- BCL2 Interacting Protein 3
- Cellular Localization
- Membrane; Mitochondrion; Mitochondrion outer membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1506MZ | Mouse Anti-BNIP3 Recombinant Antibody (clone BOb50) | ELISA, ICC, IP, WB | Mouse IgG2b |
| MRO-0187-CN | Rabbit Anti-BNIP3 Recombinant Antibody (clone JA71-10) | WB, IF, IHC | Rabbit IgG |
| VS3-WK89 | Mouse Anti-BNIP3 Recombinant Antibody (clone F7-A5) | WB, ELISA | Mouse IgG2a |
| VS3-FY163 | Recombinant Rabbit Anti-BNIP3 Antibody (clone R09-1G8) | WB, ICC, IF | Rabbit IgG |
| VS7-HM250 | Mouse Anti-BNIP3 Recombinant Antibody (clone 4E11F4) | ICC, FCM | Mouse IgG1 |
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