Mouse Anti-BRCA2 Recombinant Antibody (clone A2-B10)
CAT#: MRO-0190-CN
This antibody is a mouse monoclonal antibody that is capable of binding to BRCA2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_A_4_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26815/59973_B_2_4.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139618-BRCA2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- A2-B10
- Applications
- IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- BRCA2, DNA Repair Associated; BRCA1/BRCA2-Containing Complex, Subunit 2; Fanconi Anemia Group D1 Protein; Breast Cancer 2, Early Onset; FANCD1; FACD; Breast And Ovarian Cancer Susceptibility Gene, Early Onset; Breast And Ovarian Cancer Susceptibility Protein 2; Breast Cancer Type 2 Susceptibility Protein; Fanconi Anemia, Complementation Group D1; Breast Cancer 2 Tumor Suppressor; Truncated Breast Cancer 2;
- Gene ID
- 675
- UniProt ID
- P51587
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2502z | Mouse Anti-BRCA2 Recombinant Antibody (clone 25H11) | ICC, IF, IHC | Mouse IgG |
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| VS3-CJ1019 | Mouse Anti-BRCA2 Recombinant Antibody (VS3-CJ1019) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC106 | CytoStream™ Rabbit Anti-BRCA2 Recombinant Antibody (VS13-YC106) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY826 | Anti-BRCA2 Immunohistochemistry Kit | IHC |
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